BRCA1 Monoclonal Antibody (6B4)

货号：MA1-23164

规格：100 µL

价格：5753

产品类型：标签抗体

品牌：Thermo Fisher

抗原：BRCA1 protein fragment expressed in E. coli corres

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：6B4

荧光染料：其它

类型:	一抗	同型对照：
浓度：	2.75mg/mL	用法：	1:100-1:1000(ICC);1:100-1:1000(IF);1:500-1:3000(WB)

产品详细信息The predicted molecular weight is 220 kDa. A suggested positive control for this product is HBL100.靶标信息BRCA1 (breast and ovarian cancer susceptibility protein 1) is a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor in combination with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC (for BRCA1 associated genome surveillance complex). BRCA1 associates with RNA polymerase II, and through the C terminal domain, also interacts with histone deacetylase complex. The BRCA1 protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in the BRCA1 gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.

数据：

BRCA1 Antibody (MA1-23164) in IFImmunofluorescent analysis of BRCA1 in U2OS cells (nuclear foci induced by ionizing radiation). IR-treated (2 hr/4 gray IR) U2OS cells were pre-extracted with CSK buffer on ice for 4 min before fixation with 4% PFA in room temperature and stained using a BRCA1 monoclonal antibody (Product # MA1-23164)at a dilution of 1:400. Nuclei were stained using DAPI. Detection was performed using an Alexa Fluor-488 secondary antibody. BRCA1 Antibody (MA1-23164) in IHCImmunohistochemical analysis of BRCA1 using a BRCA1 monoclonal antibody (Product # MA1-23164). BRCA1 Antibody (MA1-23164) in WBWestern blot analysis of BRCA1 in MCF-7 and MEF (mouse embryonic fibroblast) cell lysate. Samples were resolved on a 5-15% gradient gel and probed with a BRCA1 monoclonal antibody (Product # MA1-23164).

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