Fe65 Polyclonal Antibody

货号：PA1-752

规格：100 µg

价格：5468

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Synthetic Peptide: P(352) Q E E E K L S Q R N A N

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	2 µg/mL(ICC);2 µg/mL(IF);1-2 µg/mL(WB)

产品详细信息PA1-752 detects Fe65 from purified mouse samples. This antibody does not detect endogenous levels of Fe65.PA1-752 has been successfully used in Western blot procedures. By Western blot, this antibody detects an 97 kDa protein representing Fe65 from immunopurified mouse brain lysate.The PA1-752 immunizing peptide corresponds to amino acid residues 352-369 from mouse Fe65. This peptide (Cat. # PEP-200) is available for use in neutralization and control experiments.靶标信息The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein, transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants have been described for this gene but some of their full length sequence is not known.

数据：

Fe65 Antibody (PA1-752) in IFImmunofluorescence analysis of Fe65 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Fe65 Rabbit Polyclonal Antibody (Product # PA1-752) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification. Fe65 Antibody (PA1-752) in WBWestern blot analysis was performed using tissue and membrane enriched extracts (30 µg lysate) of U-87 MG (Lane 1), SH-SY5Y (Lane 2), NTERA-2 (Lane 3), U-2 OS (Lane 4), A549 (Lane 5), Mouse Brain (Lane 6) and PC-12 (Lane 7). The blots were probed with Anti-Fe65 Rabbit Polyclonal Antibody (Product # PA1-752, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A ~ 77 kDa band corresponding to Fe65 was observed across cell lines and tissue tested. Additionally ~ 41 kDa band was observed in U-87 MG, A549, and Mouse brain. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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