FALZ Monoclonal Antibody (2F10)

货号：MA5-15842

规格：100 µL

价格：4805

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Purified recombinant fragment of human BPTF expres

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：2F10

荧光染料：其它

类型:	一抗	同型对照：
浓度：		用法：	1:200（ICC）；1:200(IF);1:500-1:2000(WB）

靶标信息BPTF (bromodomain and PHD domain transcription factor) is the largest subunit of the ATP-dependent chromatin-remodelling complex, NURF (nucleosome remodelling factor). NURF catalyses ATP-dependent nucleosome sliding and facilitates transcription. BPTF recognises histone H3 tails that are tri-methylated at K4, which marks the transcriptional start site of the vast majority of transcriptionally active genes. BPTF also exhibits some binding to H3 di-methylated at K4. BPTF plays a key role in the development of early mouse embryos, possibly through regulation of the Smad pathway of transcription factors. While BPTF is expressed in low levels in the adult brain and spinal cord, it is expressed in higher levels in the brain in neurodegenerative diseases. It is present in a subset of amyloid-containing plaques in the brains of patients suffering from Alzheimer's disease. Abundantly expressed in the fetal brain. Present throughout the gray and white matter of the developing spinal cord at 18-22 gestational weeks. Expressed at low levels in adult brain and spinal cord and reexpressed in neurodegenerative diseases (at protein level).Tissue specificity: Ubiquitously expressed, with highest levels in testis. Present in kidney, liver and brain. In the brain, highest levels are found in motor cortex (at protein level).

数据：

FALZ Antibody (MA5-15842) in IFImmunofluorescent analysis of BDTF (red) in human embryonic stem cell H9 line grown on irradiated MEF-feeder layer. The cells were fixed with 4% paraformaldehyde at room temperature for 10 min and permeabilized with 0.25% Triton-X 100 for 5 min and blocked with the 10% BSA in PBS for 30 min at 37°C. Cells were stained with a BDTF monoclonal antibody (Product # MA5-15842) at a dilution of 1:200 in 3% BSA/PBS blocking buffer overnight at 4°C, and then incubated with a RRX-conjugated donkey anti-mouse IgG secondary antibody at a dilution of 1:500 for 1 hour at room temperature. Nucleus DNA (blue) was stained with DAPI (Product # D1306). FALZ Antibody (MA5-15842) in WBWestern blot analysis of BPTF using BPTF monoclonal antibody (Product # MA5-15842) in HEK293 (1) and BPTF (AA: 503-670) human IgG Fc transfected HEK293 (2) cell lysate.

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