HP1 beta Polyclonal Antibody

货号：PA5-28448

规格：100 µL

价格：4692

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to a region within

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	1:100-1:1000（IHC(P)）；1:100-1:1000（ICC）；1:100-1:1000(IF);1:500-1:3000(WB）

产品详细信息Recommended positive controls: Raji.Predicted reactivity: Mouse (100%), Chimpanzee (100%), Bovine (100%).Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.靶标信息This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified.

数据：

HP1 beta Antibody (PA5-28448) in IFImmunofluorescent analysis of CBX1/HP1 beta in paraformaldehyde-fixed HeLa cells using a CBX1/HP1 beta polyclonal antibody (Product # PA5-28448) at a 1:200 dilution. HP1 beta Antibody (PA5-28448) in IHC (P)Immunohistochemical analysis of CBX1/HP1 beta in the nucleus of paraffin-embedded mouse ovary tissue using a CBX1/HP1 beta polyclonal antibody (Product # PA5-28448). HP1 beta Antibody (PA5-28448) in WBWestern blot was performed using Anti- HP1 Polyclonal Antibody (Product # PA5-28448) and a 25 kDa band corresponding to HP1 was observed across the cell lines along with additional bands between 60-160kDa. Modified whole cell extracts (30ug) of MCF7 (Lane 1), HeLa (Lane 2) and A-431 (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

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