CBX7 Polyclonal Antibody

货号：PA5-40905

规格：100 µL

价格：4805

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Synthetic peptide directed towards the middle regi

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	0.5 mg/mL	用法：	2.5 µg/10^6 cells（ChIP）；1:100（ICC）；1:100(IF);0.2-1 µg/mL(WB）

产品详细信息Peptide sequence: ADLAEGPPPW TPALPSSEVT VTDITANSIT VTFREAQAAE GFFRDRSGKFSequence homology: Cow: 100%; Dog: 93%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100%靶标信息The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.

数据：

CBX7 Antibody (PA5-40905) in IFImmunofluorescence analysis of CBX7 was performed using 70% confluent log phase 3T3-L1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CBX7 Polyclonal Antibody (Product # PA5-40905) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing Cytoplasmic localization. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. CBX7 Antibody (PA5-40905) in WBWestern blot was performed using Anti-CBX7 Polyclonal Antibody (Product # PA5-40905) and a 28kDa band corresponding to CBX7 was observed across cell lines and tissue tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of U-2 OS (Lane 1), LNCaP (Lane 2), A-431 (Lane 3), Hep G2 (Lane 4), HeLa (Lane 5), COLO 205 (Lane 6), HCT 116 (Lane 7) and tissue extract of Mouse Spleen (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1ug/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

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