CBX7 Recombinant Rabbit Monoclonal Antibody (JM44-63)

货号：MA5-32767

规格：100 µL

价格：4732

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Recombinant protein within human cbx7 aa 1-150

物种：人

宿主：兔

抗体亚型：IgG

克隆号：JM44-63

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	1:50-1:100（IHC(P)）；1:500-1:2,000(WB）

产品详细信息Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.

数据：

CBX7 Antibody (MA5-32767) in WBWestern blot was performed using Anti-CBX7 Recombinant Rabbit Monoclonal Antibody (JM44-63) (Product # MA5-32767) and a band at 30 kDa corresponding to CBX7 was observed across the cell lines tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of A-431 (Lane 1), Hep G2 (Lane 2), HeLa (Lane 3) and COLO 205 (Lane 4) were electrophoresed using Novex® NuPAGE® 12% % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

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