UHRF1 Polyclonal Antibody

货号：PA5-29884

规格：100 µL

价格：5547

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Recombinant fragment corresponding to a region wit

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	1:100-1:1000（IHC(P)）；1:100-1:1000（ICC）；1:100-1:1000(IF);1:100-1:500（IP）；1:500-1:3000(WB）

产品详细信息Recommended positive controls: HCT116, K562.Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.靶标信息This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encodingdifferent isoforms have been found for this gene.

数据：

UHRF1 Antibody (PA5-29884) in IFImmunofluorescent analysis of UHRF1 in paraformaldehyde-fixed HeLa cells using a UHRF1 polyclonal antibody (Product # PA5-29884) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled withProduct # PA5-29281(Red) at a 1:2000. UHRF1 Antibody (PA5-29884) in IHC (P)Immunohistochemical analysis of UHRF1 in paraffin-embedded HBL435 xenograft using a UHRF1 polyclonal antibody (Product # PA5-29884) at a dilution of 1:500. UHRF1 Antibody (PA5-29884) in WBWestern blot was performed using Anti-UHRF1 Polyclonal Antibody(Product # PA5-29884) and a 90 kDa band corresponding to UHRF1 was observed along with uncharacterized bands(*) across cell lines . Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), HeLa (Lane 2) and MDA-MB-231 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-47613.htm