14-3-3 sigma Monoclonal Antibody (1433S01)

货号：MA5-11663

规格：500 µL

价格：5836

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Recombinant human 1433 sigma protein

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：1433S01

荧光染料：其它

类型:	一抗	同型对照：
浓度：	0.2 mg/mL	用法：	2-4 µg/mL（IHC(P)）；1:10-1:200（ICC）；1:10-1:200(IF);2 µg/mL（IP）；1 µg/mL(WB）

产品详细信息MA5-11663 targets 14-3-3 sigma in ICC/IF, IHC (P), IP, and WB applications and shows reactivity with Human samples.The MA5-11663 immunogen is recombinant human 1433 sigma protein.靶标信息This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis.

数据：

14-3-3 sigma Antibody (MA5-11663) in IFImmunofluorescence analysis of 14-3-3 sigma was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled 14-3-3 sigma (1433S01) Mouse Monoclonal Antibody (Product # MA5-11663) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification. 14-3-3 sigma Antibody (MA5-11663) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (lane 1), MCF-7 (lane 2), A431 (lane 3), HT-29 (Lane 4) and HeLa (lane 5). The blots were probed with Anti-14-3-3 Sigma Mouse Monoclonal Antibody (Product # MA5-11663, 0.5-1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 28 kDa band corresponding to 14-3-3 Sigma was observed across cell line tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with PierceTM Power Blotter System (Product # 22834) The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-47617.htm