Estriol ELISA Kit ELISA试剂盒,Estriol ELISA Kit/雌三醇ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Estriol ELISA Kit ELISA试剂盒,Estriol ELISA Kit/雌三醇ELISA试剂盒

2024-10-24

Estriol ELISA Kit/雌三醇ELISA试剂盒

货号：EIAEST,EIAESTX10

规格：96 tests,10X96 tests

价格：6143,57271

产品类型：ELISA检测

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	5.33 pg/mL
检测范围	19.66-12,000 pg/mL
样本类型/体积	血清 50 µL 血浆 50 µL 粪便提取物 50 µL 尿液 12.5 µL 上清液 50 µL
操作时间	45 min
测试时间	2 hr 30 min
Homogenous (no wash)	No
批间变异系数（CV）	12%
批内变异系数（CV）	10%
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	96 Tests
组份	- 抗-lgG包被96孔板 - 标准品 - 测定稀释浓缩液 - 捕获抗体 - HRP结合抗原 - 洗涤浓缩液 - TMB底物 - 终止液 - 胶板盖
储存温度	-20°C
Protein name	Estriol
物种（Tested）	人
试剂盒类型	Competitive ELISA
标记（染料）	HRP
检测抗体偶联物	HRP

The Estriol Competitive ELISA quantitates estriol in serum, plasma, urine, saliva, dried fecal extracts or or cell culture medium.|Principle of the method: The Estriol Competitive ELISA research-use-only kit is designed to quantitatively measure estriol independent of species. An estriol standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-rabbit IgG antibodies. Anti-rabbit estriol antibodies and estriol-peroxidase conjugate is added to the wells. The estriol-peroxidase conjugate and any estriol in the sample will compete to bind to the anti-rabbit estriol antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound estriol-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of estriol present in the sample.|Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.Estriol竞争性ELISA对血清，血浆，尿液，唾液，干燥的粪便提取物或细胞培养基中的雌三醇进行定量。|方法的原理：Estriol竞争性ELISA研究专用试剂盒用于定量测量雌三醇，而与物种无关。提供雌三醇标准品以生成测定的标准曲线，并从用户生成的标准曲线中读取所有样品。将标准品或稀释的样品吸移到涂有抗兔IgG抗体的透明微量滴定板中。将抗兔雌三醇抗体和雌三醇-过氧化物酶偶联物添加至孔中。雌三醇过氧化物酶结合物和样品中的任何雌三醇都将竞争结合抗兔雌三醇抗体。温育后，洗涤板并加入底物。底物与结合的雌三醇-过氧化物酶缀合物反应。孵育30分钟后，停止反应，并在微量滴定板读数器中于450 nm处检测所产生颜色的强度。产生的颜色的强度与样品中雌三醇的含量成反比。|严格的验证：对该ELISA试剂盒的每个制造批次都进行了质量检测，测试标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息Estriol is the main etrogen in the blood and urine of pregnant females. Serum estriol dterminations reflect the status of the fetus and are used to evaluate fetal distress and placental function.雌三醇是怀孕女性血液和尿液中的主要雌激素。血清雌三醇测定值反映了胎儿的状况，并用于评估胎儿的窘迫和胎盘功能。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

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Estriol ELISA Kit ELISA试剂盒,Estriol ELISA Kit/雌三醇ELISA试剂盒

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