GeneJET Plant Genomic DNA Purification mini Kit/植物基因组DNA提取试剂盒

货号：K0791,K0792

规格：50 preps,250 preps

价格：1219,5345

产品类型：DNA提取

品牌：Thermo Fisher

Thermo Scientific GeneJET Plant Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from a wide variety of plant species and tissue types. It utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation.Highlights•Fast—procedure completed in 30 min following cell lysis•Convenient—protocol does not include clogging-prone lysate filtration step•High yield—up to 32 µg of high quality DNA from 100 mg sample, including nuclear, mitochondrial, and chloroplast DNA•Compatible—use with awide variety of samples (leaves, roots, sprouts and other parts of various plants)The standard procedure takes less than 30 minutes following cell lysis and yields purified DNA greater than 30 kb in size. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and the age of the tissue sample. The typical yield from a standard tissue source (e. g. young wheat leaves) is 30 to 32 µg from 100 mg of tissue. Isolated DNA can be used directly in PCR, qPCR, southern blotting, and enzymatic reactions.Applications• Isolated genomic DNA is ideal for use in common molecular biology procedures, including:• PCR and qPCR•FastDigestandconventional restrictiondigestion• Southern blottingPurification ProcedureGenomic DNA purified from various sources using the GeneJET Plant Genomic DNA Purification Mini Kit200ng of isolated genomic DNA from each source was fractionated by agarose gel (0.5%) electrophoresis.M – Lambda DNA/HindIII Marker (#SM0101)1 – Arabidopsis2 – Tobacco leaves3 – Tomato leaves4 – Spinach leaves5 – Wheat leaves6 – Rape leaves7 – Onion leaves8 - Rice leaves9 – Corn seeds10 – Sunflower stalk11 – Corn roots12 – Beet roots13 – Cucumber fruit14 – Sugar cane

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