Progesterone Metabolites ELISA Kit ELISA试剂盒,Progesterone Metabolites ELISA Kit/孕酮代谢物ELISA试剂盒

2024-10-24

Progesterone Metabolites ELISA Kit/孕酮代谢物ELISA试剂盒

货号:EIAPROGM

规格:96 tests

价格:6641

产品类型:ELISA检测

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

51.2 pg/mL

检测范围102.4-10,000 pg/mL

样本类型/体积

血清 50 µL

血浆 50 µL

粪便提取物 50 µL

尿液 12.5 µL

上清液 50 µL

操作时间

45 min

测试时间

2 hr 30 min

Homogenous (no wash)

No

批间变异系数(CV)7%
批内变异系数(CV)7%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

96 Tests

组份

- 抗-lgG包被96孔板

- 标准品

- 测定稀释浓缩液

- 捕获抗体

- HRP结合抗原

- 洗涤浓缩液

- TMB底物

- 终止液

- 胶板盖

储存温度

-20°C

Protein nameProgesterone Metabolites

物种(Tested)

试剂盒类型

Competitive ELISA

标记(染料)

HRP

检测抗体偶联物HRP
The Progesterone Metabolites Competitive ELISA quantitates progesterone metabolites in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.|Principle of the method: The Progesterone Metabolites Competitive ELISA research-use-only kit is designed to quantitatively measure progesterone metabolites independent of species. A progesterone metabolites standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-rabbit IgG antibodies. Anti-rabbit progesterone metabolites antibodies and progesterone metabolites-peroxidase conjugate is added to the wells. The progesterone metabolites-peroxidase conjugate and any progesterone metabolites in the sample will compete to bind to the anti-rabbit progesterone metabolites antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound progesterone metabolites-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of progesterone metabolites present in the sample.|Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.孕酮代谢物竞争性ELISA定量分析血清,血浆,尿液,唾液,干燥粪便提取物或细胞培养基中的孕酮代谢物。|方法原理:孕酮代谢物竞争性ELISA研究专用试剂盒旨在定量测量独立于孕酮的代谢物种。提供孕酮代谢物标准品以生成测定的标准曲线,并从用户生成的标准曲线中读取所有样品。将标准品或稀释的样品吸移到涂有抗兔IgG抗体的透明微量滴定板中。将抗兔孕酮代谢物抗体和孕酮代谢物-过氧化物酶结合物添加到孔中。孕酮代谢物-过氧化物酶结合物和样品中的任何孕酮代谢物将竞争结合抗兔孕酮代谢物抗体。温育后,洗涤板并加入底物。底物与结合的孕酮代谢物-过氧化物酶结合物反应。孵育30分钟后,停止反应,并在微量滴定板读数器中于450 nm处检测所产生颜色的强度。产生的颜色的强度与样品中孕酮代谢产物的含量成反比。|严格的验证:此ELISA试剂盒的每批生产均经过质量检测,测试标准包括敏感性,特异性,精密度和批次间一致性。有关验证的更多信息,请参见手册。Target information 靶标信息Progesterone, C21H30O2, also known as P4 (pregn-4-ene-3,20-dione) is a C-21 steroid hormone involved in the female menstrual cycle, gestation and embryogenesis of humans and other species. Progesterone belongs to a class of hormones called progestogens and is the major naturally occurring human progestogen. Progesterone is an essential regulator of human female reproductive function in the uterus, ovary, mammary gland and brain, and plays an important role in non-reproductive tissues such as the cardiovascular system, bone and the central nervous system.孕酮C21H30O2,也称为P4(pregn-4-ene-3,20-dione)是一种C-21类固醇激素,参与女性和其他物种的女性月经周期,妊娠和胚胎发生。孕酮属于一类称为孕激素的激素,是主要的天然人类孕激素。孕酮是人类女性在子宫,卵巢,乳腺和脑中生殖功能的重要调节剂,在非生殖组织(如心血管系统,骨骼和中枢神经系统)中起着重要作用。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。

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