CD336 (NKp44) Monoclonal Antibody (44.189), eFluor 450,

货号：48-3369-42

规格：100 Tests

市场价格：3090

产品类型：流式抗体

品牌：eBioscience

抗原：CD336 (NKp44)

物种：人

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：44.189

荧光染料：eFluor 450

类型:	单抗	同型对照：	Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor 450,
浓度：	5 µL/Test	用法：	5 µL (1 µg)/test(Flow)

产品详细信息Description: The monoclonal antibody 44.189 reacts with human CD336, which is also known as NKp44 or NCR2. CD336 is a 44-kDa transmembrane glycoprotein belonging to the natural cytotoxicity receptor (NCR) family and contains an ITIM domain located in the cytoplasmic tail. Expression of CD336 and NK cell activation by the receptor requires association with DAP12, an ITAM-containing transmembrane accessory protein. Once thought to be expressed exclusively on IL-2-activated NK cells and a subset ofgamma delta T lymphocytes, studies have shown expression of CD336 on IL-3-stimulated plasmacytoid dendritic cells, alpha beta T cells in the small intestinal epithelium, and T cells in cord blood. CD336 has been shown to be involved in the innate response of activated NK cells against viral infection and tumor cell proliferation.The 44.189 antibody has been reported to facilitate CD336 crosslinking.Applications Reported: This 44.189 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 44.189 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (1.0 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD336 is also known as NKp44 or NCR2. CD336 is a 44-kDa transmembrane glycoprotein belonging to the natural cytotoxicity receptor (NCR) family and contains an ITIM domain located in the cytoplasmic tail. Expression of CD336 and NK cell activation by the receptor requires association with DAP12, an ITAM-containing transmembrane accessory protein. Once thought to be expressed exclusively on IL-2-activated NK cells and a subset of gamma delta T lymphocytes, studies have shown expression of CD336 onIL-3-stimulated plasmacytoid dendritic cells, alpha beta T cells in the small intestinal epithelium, and T cells in cord blood. CD336 has been shown to be involved in the innate response of activated NK cells against viral infection and tumor cell proliferation.

数据

CD336 (NKp44) Antibody (48-3369-42) in FlowNormal human peripheral blood cells were stimulated with Human IL-2 Recombinant Protein (Product # 14-8029-81) for 15 days, then surface stained with Anti-Human CD56 (NCAM) FITC (Product # 11-0566-42) and Mouse IgG2b K Isotype Control eFluor® 450 (Product # 48-4732-82) (left) or Anti-Human CD336 (NKp44) eFluor® 450 (right). Viable cells in the lymphocyte gate, as determined by Fixable Viability Dye eFluor® 660 (Product # 65-0864-14), were used for analysis.

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参考文献

1. ImmunityVagal Regulation of Group 3 Innate Lymphoid Cells and the Immunoresolvent PCTR1 Controls Infection Resolution.

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