Human RANTES Uncoated ELISA Kit with Plates,Human RANTES Uncoated ELISA Kit with Plates/人RANTES未包被ELISA试剂盒含未包被板

2024-10-24

Human RANTES Uncoated ELISA Kit with Plates/人RANTES未包被ELISA试剂盒含未包被板

货号:88-50287-22,88-50287-86,88-50287-76

规格:2X96 tests,10X96 tests,20X96 tests

价格:3076,6115,8481

产品类型:自定义ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

<46.9 pg/mL

检测范围46.9-3000 pg/mL

样本类型/体积

血清 50 μL

血浆 50 μL

上清液 50 μL

操作时间

1 hr 45 min

测试时间

24 hr 30 min

Homogenous (no wash)

No

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

20X 96 Tests

组份

- 捕获抗体:Pre-titrated, 纯化抗体

- 检测抗体:Pre-titrated, Biotin偶联抗体

- 标准品:重组细胞因子,用于生成标准曲线和校准样品

- 10X包被缓冲液:用于包被捕获抗体的缓冲液

- 检测酶:Pre-titrated Avidin-HRP浓缩液(100X)

-分析缓冲液A

- 底物溶液:T1XTMB 底物溶液

- 分析证书:特定批次说明书用于抗体和标准品稀释

- 96孔板:Corning Costar 9018(含产品目录号,后缀 -22,-76,-86)

储存温度

2-8℃

Protein nameCCL5 (RANTES)
Protein aliasesbeta-chemokine RANTES, C-C motif chemokine 5, chemokine (C-C motif) ligand 5, EoCP, Eosinophil chemotactic cytokine, RANTES(3-68), RANTES(4-68), regulated upon activation, normally T-expressed, and presumably secreted, SIS-delta, small inducible cytokine subfamily A (Cys-Cys), member 5, Small-inducible cytokine A5, T cell-specific protein P228, T-cell specific protein p288, T-cell-specific protein RANTES, TCP228

物种(Tested)

试剂盒类型

Uncoated ELISA Kitwith Plates

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesCCL5, D17S136E, eoCP, RANTES, SCYA5, SIS-delta, SISd, TCP228
Gene ID(Human) 6352
Gene symbolCCL5
UniProt ID(Human) P13501
The Human RANTES Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human RANTES. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.人RANTES未包被ELISA试剂盒含预先匹配的抗体对,以及用于执行定量酶联免疫吸附测定(ELISA)的试剂,以检测和定量人RANTES的蛋白质水平。需要洗涤液和终止液才能完成ELISA反应,需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA设计用于测量匹配的抗体对之间结合的靶标的量。将靶标特异性抗体包被在微孔板孔的底部,这是一整夜的过程。然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样品中存在的靶标浓度成正比。Target information 靶标信息RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.RANTES是血液单核细胞,记忆T辅助细胞和嗜酸性粒细胞的趋化因子。它引起嗜碱性粒细胞释放组胺并激活嗜酸性粒细胞。它与CCR1,CCR3,CCR4和CCR5结合,是CD8 + T细胞产生的主要HIV抑制因子之一。重组RANTES蛋白诱导不同株的HIV 1,HIV 2和猿猴免疫缺陷病毒(SIV)的剂量依赖性抑制。它是T细胞和巨噬细胞特有的,由有丝分裂原诱导,属于内分泌β(趋化因子CC)家族。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

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