Human GROa Uncoated ELISA Kit ELISA试剂盒,Human GROa Uncoated ELISA Kit/人GROa未包被ELISA试剂盒

2024-10-24

Human GROa Uncoated ELISA Kit/人GROa未包被ELISA试剂盒

货号:88-52122-88,88-52122-77

规格:10X96 tests,20X96 tests

价格:6096,7123

产品类型:ELISA检测

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

15.6 pg/mL

检测范围15.6-1000 pg/mL

样本类型/体积

血清 50 μL

血浆 50 μL

上清液 50 μL

操作时间

1 hr 45 min

测试时间

24 hr 30 min

Homogenous (no wash)

No

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

20X 96 Tests

组份

- 捕获抗体:Pre-titrated, 纯化抗体

- 检测抗体:Pre-titrated, Biotin偶联抗体

- 标准品:重组细胞因子,用于生成标准曲线和校准样品

-10X包被缓冲液:用于包被捕获抗体的缓冲液

- 检测酶:Pre-titrated Avidin-HRP浓缩液(100X)

- 样品稀释液Z

- 分析缓冲液A

- 底物溶液:1XTMB 底物溶液

- 分析证书:特定批次说明书用于抗体和标准品稀释

- 96孔板:Corning Costar 9018(含产品目录号,后缀 -22,-76,-86)

储存温度

2-8℃

Protein nameCXCL1 (GRO alpha)
Protein aliasesC-X-C motif chemokine 1, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha), fibroblast secretory protein, GRO-alpha(1-73), GRO-alpha(4-73), GRO-alpha(5-73), GRO-alpha(6-73), GRO1 oncogene (melanoma growth stimulating activity, GRO1 oncogene (melanoma growth-stimulating activity), Growth-regulated alpha protein, melanoma growth stimulating activity, alpha, Melanoma growth stimulatory activity, melanoma growth stimulatory activity alpha, MGSA, MGSA alpha, NAP-3, Neutrophil-activating protein 3

物种(Tested)

试剂盒类型

Uncoated ELISA Kitwith Plates

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesCXCL1, FSP, GRO, GRO1, GROA, MGSA, MGSA-a, NAP-3, SCYB1
Gene ID(Human) 2919
Gene symbolCXCL1
UniProt ID(Human) P09341
The Human GRO alpha Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human GRO alpha. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.人GRO alpha未包被ELISA试剂盒包含预先匹配的抗体对,以及用于执行定量酶联免疫吸附测定(ELISA)的试剂,以检测和定量人GRO alpha的蛋白质水平。需要洗涤液和终止液才能完成ELISA反应,需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA设计用于测量匹配的抗体对之间结合的靶标的量。将靶标特异性抗体包被在微孔板孔的底部,这是一整夜的过程。然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样本中存在的目标浓度成正比。Target information 靶标信息CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.CXCL1,CXCL2和CXCL3,也分别称为GRO alpha,GRO beta(MIP-2 alpha)和GRO gamma(MIP-2 beta),是趋化因子CXC亚家族的成员。成熟的CXCL1 / 2/3蛋白与B型IL-8受体具有高亲和力,是有效的嗜中性粒细胞引诱剂和激活剂。在体外,加工后的形式GRO-alpha(4-73),GRO-alpha(5-73)和GRO-alpha(6-73)显示出30倍高的趋化活性。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

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