Rat IL-10 Uncoated ELISA Kit ELISA试剂盒,Rat IL-10 Uncoated ELISA Kit/大鼠IL-10未包被ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rat IL-10 Uncoated ELISA Kit ELISA试剂盒,Rat IL-10 Uncoated ELISA Kit/大鼠IL-10未包被ELISA试剂盒

2024-10-24

Rat IL-10 Uncoated ELISA Kit/大鼠IL-10未包被ELISA试剂盒

货号：88-50629-88,88-50629-77

规格：10X96 tests,20X96 tests

价格：6096,7981

产品类型：自定义ELISA

品牌：Thermo Fisher

物种：大鼠

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	<15.6 pg/mL
检测范围	15.6-1000 pg/mL
样本类型/体积	血清 50 μL 血浆 50 μL 上清液 50 μL
操作时间	1 hr 45 min
测试时间	24 hr 30 min
Homogenous (no wash)	No
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	20X 96 Tests
组份	- 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 -10X包被缓冲液：用于包被捕获抗体的缓冲液 - 检测酶：Pre-titrated Avidin-HRP浓缩液（100X） - 样品稀释液Z - 分析缓冲液A - 底物溶液：1XTMB 底物溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar 9018（含产品目录号，后缀 -22，-76，-86）
储存温度	2-8℃
Protein name	IL-10
Protein aliases	CSIF, Cytokine synthesis inhibitory factor, IL-10, Interleukin-10
物种（Tested）	大鼠
试剂盒类型	Uncoated ELISA Kitwith Plates
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	Il-10, Il10, IL10X
Gene ID	(Rat) 25325
Gene symbol	Il10
UniProt ID	(Rat) P29456

The Rat Interleukin-10 (IL-10) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of rat IL-10. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.大鼠白介素10（IL-10）未包被的ELISA试剂盒包含预先匹配的抗体对，以及用于进行定量酶联免疫吸附测定（ELISA）的试剂，以检测和定量大鼠IL-10的蛋白质水平。需要洗涤液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA设计用于测量匹配的抗体对之间结合的靶标的量。将靶标特异性抗体包被在微孔板孔的底部，这是一整夜的过程。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样本中存在的目标浓度成正比。Target information 靶标信息Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.白介素10（IL-10，CSIF）是一种主要由巨噬细胞和Th2细胞产生的抗炎细胞因子。 IL-10是一个160个氨基酸残基的18.6 kDa蛋白，与爱泼斯坦-巴尔病毒蛋白（BCRFI）具有80％以上的序列同源性。 IL-10的生物学活性可能相互关联，包括抑制巨噬细胞介导的细胞因子合成，抑制迟发型超敏反应以及刺激Th2细胞反应，从而导致抗体产生增加。 IL-10通过抑制巨噬细胞和调节性T细胞（包括IFN-γ，TNF-α，IL-2和IL-3，IL-4和GM-CSF）产生的促炎细胞因子发挥功能。还已知IL-10抑制抗原呈递细胞上的抗原呈递，提高B细胞存活，增殖和抗体产生。 IL-10可以阻断NF-κB的活性，并参与JAK-STAT信号通路的调节。虽然人IL-10可以作用于鼠细胞，但是鼠IL-10不能作用于人细胞。与细胞视黄醇结合蛋白II启动子（CRBPII-RXRE）的类视黄醇X受体（RXR）响应元件结合。 IL-10抑制视黄酸响应元件的9-顺式视黄酸依赖性RXRα转录激活。小鼠的基因敲除研究表明，这种细胞因子在肠道中是必需的免疫调节剂。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

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Rat IL-10 Uncoated ELISA Kit ELISA试剂盒,Rat IL-10 Uncoated ELISA Kit/大鼠IL-10未包被ELISA试剂盒

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