Rat IL-6 Uncoated ELISA Kit ELISA试剂盒,Rat IL-6 Uncoated ELISA Kit/大鼠IL-6未包被ELISA试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rat IL-6 Uncoated ELISA Kit ELISA试剂盒,Rat IL-6 Uncoated ELISA Kit/大鼠IL-6未包被ELISA试剂盒

2024-10-24

Rat IL-6 Uncoated ELISA Kit/大鼠IL-6未包被ELISA试剂盒

货号：88-50625-88,88-50625-77

规格：10X96 tests,20X96 tests

价格：6000,6923

产品类型：自定义ELISA

品牌：Thermo Fisher

物种：大鼠

宿主：其它

抗体亚型：其它

荧光染料：其它

灵敏度	<46.9 pg/mL
检测范围	46.9-3000 pg/mL
样本类型/体积	血清 50 μL 血浆 50 μL 上清液 50 μL
操作时间	1 hr 45 min
测试时间	24 hr 30 min
Homogenous (no wash)	No
仪器设备	Colorimetric Microplate Reader 酶标仪
规格	20X 96 Tests
组份	- 捕获抗体：Pre-titrated, 纯化抗体 - 检测抗体：Pre-titrated, Biotin偶联抗体 - 标准品：重组细胞因子，用于生成标准曲线和校准样品 -10X包被缓冲液：用于包被捕获抗体的缓冲液 - 检测酶：Pre-titrated Avidin-HRP浓缩液（100X） - 分析缓冲液A - 底物溶液：1XTMB 底物溶液 - 分析证书：特定批次说明书用于抗体和标准品稀释 - 96孔板：Corning Costar 9018（含产品目录号，后缀 -22，-76，-86）
储存温度	2-8℃
Protein name	IL-6
Protein aliases	IL-6, Interleukin 6 (interferon, beta 2), Interleukin-6
物种（Tested）	大鼠
试剂盒类型	Uncoated ELISA Kitwith Plates
标记（染料）	HRP
检测抗体偶联物	Biotin
Gene aliases	Ifnb2, Il-6, Il6, ILg6
Gene ID	(Rat) 24498
Gene symbol	Il6
UniProt ID	(Rat) P20607

The Rat Interleukin-6 (IL-6) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of rat IL-6. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.大鼠白介素6（IL-6）未包被的ELISA试剂盒包含预先匹配的抗体对，以及用于进行定量酶联免疫吸附测定（ELISA）的试剂，以检测和定量大鼠IL-6的蛋白质水平。需要洗涤液和终止液才能完成ELISA反应，需单独购买。Principle of the method 方法原理ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.ELISA设计用于测量匹配的抗体对之间结合的靶标的量。将靶标特异性抗体包被在微孔板孔的底部，这是一整夜的过程。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样本中存在的目标浓度成正比。Target information 靶标信息Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.白介素6（IL-6）是一种多功能的26 kD蛋白，最初是在RNA刺激的成纤维细胞样细胞的培养基中发现的。 IL-6似乎直接参与感染和细胞损伤后发生的反应，它可能被证明在调节急性期反应中作为IL-1和TNF-a很重要。据报道IL-6由成纤维细胞，活化的T细胞，活化的单核细胞或巨噬细胞和内皮细胞产生。 IL-6作用于多种细胞，包括骨髓祖细胞，T细胞，B细胞和肝细胞。另外，IL-6似乎在T淋巴细胞的增殖中与IL-2相互作用。 IL-6增强了IL-3对多能造血祖细胞的增殖作用。 IL-6在B细胞向浆细胞的分化中起关键作用，并且是浆细胞瘤和骨髓瘤的有效生长因子。 IL-6是一种非常有用的培养补充剂，可用于产生大量产生抗体的杂交瘤。 IL-6主要在急性和慢性炎症部位产生，分泌到血清中并通过白介素6受体α诱导转录炎症反应。 IL-6的功能涉及多种与炎症相关的疾病状态，包括糖尿病和全身性青少年类风湿性关节炎。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

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Rat IL-6 Uncoated ELISA Kit ELISA试剂盒,Rat IL-6 Uncoated ELISA Kit/大鼠IL-6未包被ELISA试剂盒

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