| 抗体类型 | 单抗 | 同型对照： | Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor 450, eBioscience™ |
| 浓度： | 0.2 mg/mL | 用法： | 0.125 µg/test(Flow) |
| 产品详细信息Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170 kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions.Applications Reported: This M1/70 antibody has been reported for use in flow cytometric analysis.Applications Tested: This M1/70 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus. |
| 数据 |
| CD11b Antibody (48-0112-82) in FlowStaining of BALB/c bone marrow cells with 0.06 µg of Anti-Mouse CD11b eFluor® 450 (Product # 48-0112-82). Cells in the lymphoid (blue histogram) and myeloid (purple histogram) gates were used for analysis. |
| 推荐产品： |
| 二抗荧光二抗免疫组化一抗标签抗体 |
| 参考文献： |
| 1.OncoimmunologyThe Interleukin (IL)-1R1 pathway is a critical negative regulator of PyMT-mediated mammary tumorigenesis and pulmonary metastasis."48-0112 was used in Flow cytometry/Cell sorting to genetically investigate the role of the interleukin-1 receptor 1 pathway in breast cancer tumourigenesis and metastasis using a mouse model."AuthorsDagenais M,Dupaul-Chicoine J,Douglas T,Champagne C,Morizot A,Saleh M2.Frontiers in immunologyMicroRNA-155 Protects Group 2 Innate Lymphoid Cells From Apoptosis to Promote Type-2 Immunity."48-0112 was used in Flow cytometry/Cell sorting to identify a subset of micro-RNAs that are regulated upon group-2 innate lymphoid cells (ILC2) activation, and establish a specific role for miR-155 in regulating ILC2 survival following activation."AuthorsKnolle MD,Chin SB,Rana BMJ,Englezakis A,Nakagawa R,Fallon PG,Git A,McKenzie ANJ3.Frontiers in immunologyProapoptotic BIM Impacts B Lymphoid Homeostasis by Limiting the Survival of Mature B Cells in a Cell-Autonomous Manner."48-0112 was used in Flow cytometry/Cell sorting to indicate that BIM regulates B cell homeostasis predominantly by limiting the life span of non-activated mature B cells."AuthorsLiu R,King A,Bouillet P,Tarlinton DM,Strasser A,Heierhorst J4.Nature communicationsFat-associated lymphoid clusters control local IgM secretion during pleural infection and lung inflammation."48-0112 was used in Immunofluorescence to reveal a link between inflammation, IL-33 release by FALC stromal cells, ILC2 activation and pleural B-cell activation in FALCs, resulting in local and antigen-specific IgM production."AuthorsJackson-Jones LH,Duncan SM,Magalhaes MS,Campbell SM,Maizels RM,McSorley HJ,Allen JE,Bénézech C5.The journal of histochemistry and cytochemistry : official journal of the Histochemistry SocietyImmunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.AuthorsWhiteland JL,Nicholls SM,Shimeld C,Easty DL,Williams NA,Hill TJ |
QQ:1749072012