Miltenyi Viobility™ Fixable Dyes,Viobility™ Fixable Dyes-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Miltenyi Viobility™ Fixable Dyes,Viobility™ Fixable Dyes

2024-10-24

Viobility™ Fixable Dyes

货号：130-109-814

规格：for 100 tests

价格：0

产品类型：抗体和染料

品牌：Miltenyi

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

OverviewThe Viobility™ Fixable Dyes allow the discrimination between live and apoptotic or dead cells by flow cytometry. The dyes are suitable for both fixed and unfixed cells.Following reagents are available, addressing different fluorescent channels:

Viobility 405/452 Fixable Dye (Ex.: 405 nm, Em.: 452 nm)
Viobility 405/520 Fixable Dye (Ex.: 405 nm, Em.: 520 nm)
Viobility 488/520 Fixable Dye (Ex.: 488 nm, Em.: 520 nm)

Detailed product informationThe Viobility™ Fixable Dyes have been developed for the detection and discrimination of dead cells. These dyes react with the primary amine groups of proteins which can be found on the cell surface as well as intracellularly.In contrast to viable cells, dead cells exhibit a compromised cell membrane allowing the Viobility Fixable Dyes to enter the cell and stain proteins within the cell. This results in a up to 50-fold brighter fluorescent staining and thus allows the discrimination between dead and viable cells by using a flow cytometer.Viobility Fixable Dyes show certain advantages over traditional dead cell–staining dyes like propidium iodide (PI):

non-toxic to cells
less spillover into other fluorescent channels
more flexibility in antibody panel design, as dyes are available for various excitation and emission wavelengths

数据：

Cells stained with propidium iodide (A) or Viobility Fixable Dye (B). Cells in light orange represent apoptotic and dead cells.A mixture of live and heat-treated (for 10 minutes at 95 °C) human peripheral blood mononuclear cells (PBMCs) were stained with propidium iodide (A) or propidium iodide and Viobility 488/520 Fixable Dye (B) and analyzed by flow cytometry using the MACSQuant

图3. 在mTeSR™1中培养的人胚胎干细胞具有多能性H9 hESCs在mTeSR™1中传代6次后皮下注射到免疫缺陷小鼠体内。所得的畸胎瘤包含了3个胚层的所有细胞类型。显示典型组织类型。

参考文献：

1.Scheffold, A: et al. (2018) CD137+CD154- Expression As a Regulatory T Cell (Treg)-Specific Activation Signature for Identification and Sorting of Stable Human Tregs from In Vitro Expansion Cultures. Front Immunol 9

技术参数

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Miltenyi Viobility™ Fixable Dyes,Viobility™ Fixable Dyes

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