Thermo fisher MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), FITC,MHC Class II (I-A/I-E) Monoclonal Antibody (M5/1-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Thermo fisher MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), FITC,MHC Class II (I-A/I-E) Monoclonal Antibody (M5/1

2024-10-24

MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), FITC

货号：11-5321-81,11-5321-82,11-5321-85

规格：50μg,100μg,500μg

价格：838,1674,2947

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：M5/114.15.2

荧光染料：FITC

抗体类型	单抗	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), FITC
浓度：	0.5 mg/mL	用法：	0.125 µg/test (Flow)
产品详细信息Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.Applications Reported: M5/114.15.2 has been reported for use in flow cytometric analysis.Applications Tested: The M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long
数据
MHC Class II (I-A/I-E) Antibody (11-5321-82) in FlowStaining of C57BL/6 splenocytes with Anti-Mouse CD3e APC (Product # 17-0031-82) and 0.06 µg of Rat IgG2b K Isotype Control FITC (Product # 11-4031-82) (left) or 0.06 µg of Anti-Mouse MHC Class II (I-A/I-E) FITC (right). Cells in the lymphocyte gate were used for analysis.
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Thermo fisher MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), FITC,MHC Class II (I-A/I-E) Monoclonal Antibody (M5/1

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