eBioscience CD86 (B7-2) Monoclonal Antibody (GL1), FITC,CD86 (B7-2) Monoclonal Antibody (GL1), FITC-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

eBioscience CD86 (B7-2) Monoclonal Antibody (GL1), FITC,CD86 (B7-2) Monoclonal Antibody (GL1), FITC

2024-10-24

CD86 (B7-2) Monoclonal Antibody (GL1), FITC

货号：11-0862-81,11-0862-82,11-0862-85

规格：50μg,100μg,500μg

价格：838,1649,2788

产品类型：流式抗体

品牌：Thermo Fisher

抗原：CD86

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：GL1

荧光染料：FITC

抗体类型	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), FITC
浓度：	0.5 mg/mL	用法：	0.125 µg/test (Flow)
产品详细信息Description: The GL1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). This interaction plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation, supports its major contribution during the primary phase of an immune response.Applications Reported: The GL1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This GL1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is theligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis
数据
CD86 (B7-2) Antibody (11-0862-82) in FlowStaining of LPS-stimulated splenocytes with Anti-Mouse CD86 (B7-2) FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
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参考文献：
1.Frontiers in immunologyMutation of an L-Type Calcium Channel Gene Leads to T Lymphocyte Dysfunction."Published figure using CD86 (B7-2) monoclonal antibody (Product # 11-0862-82) in Flow Cytometry"2.Frontiers in cellular neuroscienceTemporal Characterization of Microglia/Macrophage Phenotypes in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury."Published figure using CD86 (B7-2) monoclonal antibody (Product # 11-0862-82) in Flow Cytometry"3.Frontiers in pharmacologyGinsenoside Rg3 Mitigates Atherosclerosis Progression in Diabetic apoE-/- Mice by Skewing Macrophages to the M2 Phenotype."Published figure using CD86 (B7-2) monoclonal antibody (Product # 11-0862-82) in Flow Cytometry"4.Frontiers in cellular neuroscienceTemporal Characterization of Microglia/Macrophage Phenotypes in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury."Published figure using CD86 (B7-2) monoclonal antibody (Product # 11-0862-82) in Flow Cytometry"5.Frontiers in cellular neuroscienceTemporal Characterization of Microglia/Macrophage Phenotypes in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury."Published figure using CD86 (B7-2) monoclonal antibody (Product # 11-0862-82) in Flow Cytometry"

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