pUC19 DNA

货号：SD0061

规格：50μg

价格：609

产品类型：质粒

品牌：Thermo Fisher

描述Thermo Scientific pUC19 vector is a small, high copy number,E. coliplasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC18 vector except that it is arranged in opposite orientation.Highlights• Purified by chromatography using proprietary patented technology• More than 90% in the supercoiled form• Isolated fromE.coli(dam+,dcm+)• For pUC18 DNA sequence, pUC19 DNA sequence, sequence analysis and map creation, seefree online REviewer tool.Applications• Cloning• Sequencing of insert DNA, pUC18 DNA: Preparation of DNA molecular weight standardspUC18/19 plasmid contents and usage notes - pUC18/19 plasmids contain:• The pMB1 repliconrepresponsible for the replication of plasmid (source – plasmidpBR322). The high copy number of pUC plasmids is a result of the lack of theropgene and a single point mutation in the repliconrepof pMB1.• Theblagene, encoding beta-lactamase, confers resistance to ampicillin (source – plasmid pBR322). It differs from that of pBR322 by two point mutations• The region ofE.coli lacoperon containing a CAP protein binding site, promoter Plac,lacrepressor binding site and the 5’-terminal part of thelacZgene encoding the N-terminal fragment of beta-galactosidase (source – M13mp18/19). This fragment, whose synthesis can be induced by IPTG, is capable of intra-allelic (alpha) complementation with a defective form of beta-galactosidase encoded by the host (mutation delta(lacZ)M15). In the presence of IPTG, bacteria synthesize both fragments of the enzyme and form blue colonies on media with X-Gal. Insertion of DNA into the MCS located within thelacZgene (codons 6-7 oflacZare replaced by MCS) inactivates the N-terminal fragment of beta-galactosidase and abolishes alpha-complementation. Bacteria carrying recombinant plasmids therefore give rise to white coloniesThe map shows enzymes that cut pUC18 DNA once. Enzymes produced by Thermo Scientific are shown in orange. The coordinates refer to the position of the first nucleotide in each recognition sequence.The exact positions of the genetic elements are shown on the map (termination codons included). Theblagene nucleotides 2486-2418 (complementary strand) code for a signal peptide. The LacZ polypeptide corresponding to wt beta-galactosidase and essential for blue/white screening ends at nt position 236 (compl. strand). Another 30 codons in the same reading frame are derived from pBR322. The indicatedrepregion is sufficient to promote replication. DNA replication initiates at position 866 (± 1) and proceeds in the direction indicated. Plasmids carrying the pMB1 and ColE1 replicons are incompatible, but they are fully compatible with those carrying the p15A replicon (pACYC177, pACYC184). pMB1-derived plasmids can be amplified using chloramphenicol.Related ProductspUC18 DNApUC57 DNA

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-47930.htm