pTZ19R DNA

货号：SD0141

规格：50μg

价格：969

产品类型：质粒

品牌：Thermo Fisher

描述Thermo Scientific pTZ19R/U are small phagemids 2862 bp in length, constructed by inserting the DNA of phage f1 intergenic region (IG) intopUC19and the T7 promoter sequence near to the MCS of pUC19. The phagemids differ in the orientation of the cloned f1 IG region. They are designed for DNA cloning, dideoxy DNA sequencing,in vitromutagenesis, andin vitrotranscription in one system. A host strain harboring these phagemids will produce single-stranded DNA if superinfected with the helper phage M13K07.Highlights• Isolated fromE. coli(dam+, dcm+)• More than 90% in the supercoiled form• Isolated fromE.coli(dam+,dcm-)• For DNA sequence, sequence analysis and map creation seefree online REviewer tool.Applications• Cloning• Sequencing of insert DNAin vitrotranscriptionpTZ19R contents and usage notes - pTZ19R/U phagemids contain:• The pMB1 repliconrepresponsible for the replication of phagemid (source – plasmid pUC19)• Theblagene, coding for beta-lactamase, that confers resistance to ampicillin (source – plasmid pUC19)• The region ofE. colioperonlaccontaining a CAP protein binding site, promoter Plac,lacrepressor binding site and the 5’-terminal part oflacZgene encoding the N-terminal fragment of beta-galactosidase (source – pUC19). This fragment allows blue/white screening of recombinant phagemids in the same way as described in the pUC18/19 section• A T7 promoter inserted near to the MCS of pUC19, allowingin vitrosynthesis of large amounts of specific RNA• The phage f1 intergenic region carrying the sequences required incisfor initiation and termination of phage f1 DNA synthesis (+ and - strands) and for packaging DNA into bacteriophage particlesSynthesis of single-stranded (plus) DNA requires phage-encoded gene II, X, and V proteins. It is initiated at ori (+) and proceeds in the direction indicated. The conversion of plus DNA strands to double-stranded DNA does not require any of the phage genes. DNA synthesis is initiated by a 30-nucleotide RNA primer synthesized by the host’s RNA polymerase and starting at ori (-).The map shows enzymes that cut pTZ19R DNA once. Enzymes produced by Thermo Scientific are shown in orange. The coordinates refer to the position of the first nucleotide in each recognition sequence.The exact positions of the genetic elements are shown on the map (termination codons included). Theblagene nucleotides 2732-2664 (complementary strand) code for a signal peptide. The LacZ polypeptide corresponding to wt beta-galactosidase and essential for blue/white screening ends at nt position 458 (complementary strand). The remaining amino acids in the LacZ reading frame are encoded by f1 DNA. The indicatedrepregion is sufficient to promote replication. DNA replication initiates in the complementary DNA strand at position 1112 (± 1) and proceeds in the direction indicated. Phagemids and plasmids carrying the pMB1 and ColE1 replicons are incompatible with one another, but are fully compatible with those carrying p15A replicon (pACYC177, pACYC184). pMB1-derived plasmids can be amplified using chloramphenicol.

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-47938.htm