DNA内切酶，thermo,HphI（AsuHPI） (10 U/µL)/HphI内切酶-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

DNA内切酶，thermo,HphI（AsuHPI） (10 U/µL)/HphI内切酶

2024-10-24

HphI（AsuHPI） (10 U/µL)/HphI内切酶

货号：ER1101,ER1102

规格：300 units,300 units

价格：676,2664

产品类型：内切酶

品牌：Thermo Fisher

Thermo Scientific HphI restriction enzyme recognizes GGTGA(8/7)^ sites and cuts best at 37°C in B buffer. SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Isoschizomers: AsuHPI.Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.Note:Assayed using lambda DNA (dam-) (#SD0021). HphI is blocked by overlapping dam methylation. To avoid dam methylation, use a dam-, dcm- strain, such as GM2163. High glycerol (>5%) concentrations, pH >8.0 or a large excess of enzyme may result in star activity. For methylation sensitivity, refer to product specifications.Thermo Scientific HphI限制性内切酶可识别GGTGA（8/7）^位点，并在37°C的B种缓冲液中切割效果最佳。有关此酶及其他限制酶的酶活性，双重消化条件和热灭活条件，请参见限制酶的反应条件表格。注意：也可以作为FastDigest酶用于快速DNA消化。同分异构体：AsuHPI。Thermo Scientific常规限制性内切核酸酶是大量高质量的限制性内切酶，经过优化可在五种缓冲液系统的一种缓冲液中工作。此外，通用的Tango缓冲液为双消化提供了便利。在推荐的缓冲液和反应条件下，所有酶均具有100％的活性。为确保性能稳定，Thermo Scientific限制性内切酶反应缓冲液包含预混合的BSA，可增强许多酶的稳定性并结合DNA制备物中可能存在的污染物。注意：使用lambda DNA（dam-）（＃SD0021）进行分析。 HphI被重叠的dam甲基化所阻断。为避免dam甲基化，请使用dam-，dcm-菌株，例如GM2163。高甘油（> 5％）浓度，pH> 8.0或大量过量的酶可能会导致星号活性。有关甲基化敏感性，请参阅产品说明。

优点：

• Superior quality—stringent quality control and industry leading manufacturing process• Convenient color-coded Five Buffer System• Includes universal Tango buffer for double-digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificities• 优异的质量—严格的质量控制和业内领先的生产流程• 使用颜色标记的五大便捷缓冲系统• 包含适于双酶切的通用型Tango缓冲液• 反应缓冲液中预先混入BSA• 种类齐全，特异性限制性内切酶的广泛选择

数据：

技术参数

产品优点 • Superior quality—stringent quality control and industry leading manufacturing process • Convenient color-coded Five Buffer System • Includes universal Tango buffer for double-digestions • BSA premixed in reaction buffers • Wide selection of restriction endonuclease specificities • 优异的质量—严格的质量控制和业内领先的生产流程 • 使用颜色标记的五大便捷缓冲系统 • 包含适于双酶切的通用型Tango缓冲液 • 反应缓冲液中预先混入BSA • 种类齐全，特异性限制性内切酶的广泛选择

产品应用 • Molecular cloning • Restriction site mapping • Genotyping • Southern blotting • Restriction fragment length polymorphism (RFLP) • SNP •分子克隆 •限制性位点比对 •基因分型 •Southern印迹 •限制性片段长度多态性（RFLP） •SNP（单核苷酸多态性）

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DNA内切酶，thermo,HphI（AsuHPI） (10 U/µL)/HphI内切酶

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