F124L Thermo PCR,Phire Green Hot Start II DNA Polymerase-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

F124L Thermo PCR,Phire Green Hot Start II DNA Polymerase

2024-10-24

Phire Green Hot Start II DNA Polymerase

货号：F124L,F124S

规格：1000反应,200 reactions

价格：5654,1490

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared toTaqDNA polymerase.The hot start modification of Phire Hot Start II DNA Polymerase is based on the Affibody inactivation technology. This technology increases the specificity of the PCR reaction with no additional time required for initial activation of the enzyme.Phire Green format is a combination of Phire Hot Start II DNA Polymerase and 5X Phire Green Buffer. The buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.Note:The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that ofTaq-based polymerases. For optimal results start by accurately calculating your Tm with ourTm calculator.

优点：

-Quick hot start—No reactivation step-Fast enzyme—Amplify four times faster than with hot startTaq-Robust—Minimal reaction optimization due to high inhibitor tolerance-High yields—Abundant products due to high efficiency-Longer PCR products—Amplify significantly longer DNA fragments than with any hot startTaq- Direct loading on gel with Green Buffer

数据：

图1.Phire Hot Start II amplifies longer fragments than any hot startTaqFive genomic DNA fragments of different lengths were amplified with three different hot start DNA polymerases. Phire Hot Start II DNA Polymerase produced all five amplicons with very high yields. The competing hot startTaqDNA polymerases produced significantly lower yields and failed to amplify the 7.5kb fragment.1 – 0.6kb fragment of human glutathione peroxidase 3 gene2 – 1.0kb fragment of human glutathione peroxidase 3 gene3 – 1.5kb fragment of human cathepsin K gene4 – 2.7 kb fragment of human ß-2-microglobulin gene5 – 7.5 kbfragment of human ß-globin gene

图2.Superior yields in significantly shorter timeA 1.5kb fragment from the human cathepsin K gene was amplified with five different hot start DNA polymerases. Phire Hot Start II DNA Polymerase amplified high amounts of specific PCR product in just 29minutes. In contrast, the PCR protocols for hot startTaqDNA polymerases from four major suppliers (A through D) were substantially longer and resulted in lower product yields.

组成成分：

▪1 x 1 mL Phire Hot Start II DNA Polymerase▪7 x 1.5 mL 5X Phire Green Reaction Buffer (provides 1.5 mM MgCl2)▪ 1 x 500 µL DMSO

F124L Thermo PCR,Phire Green Hot Start II DNA Polymerase

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