DNA内切酶，thermo,SgeI (3 U/µL)/SgeI内切酶-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

DNA内切酶，thermo,SgeI (3 U/µL)/SgeI内切酶

2024-10-24

SgeI (3 U/µL)/SgeI内切酶

货号：ER2211

规格：250 units

价格：4223

产品类型：内切酶

品牌：Thermo Fisher

SgeI cleaves DNA targets containing 5-methylcytosine on one or both DNA strandsThermo Scientific SgeI restriction enzyme recognizes m5CNNG(9/13)^ sites and cuts best at 37°C in its own unique buffer. SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.Note:DNA methylated by Dcm or CpG methyltransferases will be a substrate for SgeI. Greater than 3-fold overdigestion with SgeI may result in incomplete cleavage. At least two copies of SgeI recognition sequence are required for an efficient cleavage. Amount of the enzyme required for complete digestion of methylated DNA depends on the number of SgeI recognition sites. DNA cleavage products generated by target site cleavage facilitate the nonspecific cleavage by SgeI. Therefore, optimization of the enzyme amount is recommended for DNA cleavage. pBR322 DNA isolated from E. coli dcm+ strain (#SD0041) can be used as a DNA cleavage efficiency control. SgeI cleaves all six dcm methylated targets on pBR322 DNA. For methylation sensitivity, refer to product specifications.SgeI切割一条或两条DNA链上含有5-甲基胞嘧啶的DNA靶标。Thermo Scientific SgeI限制性内切酶识别m5CNNG（9/13）^位点，并在其配套的缓冲液中于37°C切割效果最佳。有关此酶及其他限制酶的酶活性，双重消化条件和热灭活条件，请参见限制酶的反应条件表格。

Thermo Scientific常规限制性内切核酸酶是大量高质量的限制性内切酶，经过优化可在五种缓冲液系统的一种缓冲液中工作。此外，通用的Tango缓冲液为双消化提供了便利。在推荐的缓冲液和反应条件下，所有酶均具有100％的活性。为确保性能稳定，Thermo Scientific限制性内切酶反应缓冲液包含预混合的BSA，可增强许多酶的稳定性并结合DNA制备物中可能存在的污染物。注意：被Dcm或CpG甲基转移酶甲基化的DNA将成为SgeI的底物。超过3倍的SgeI过量消化可能导致不完全切割。有效裂解需要至少两个拷贝的SgeI识别序列。完全消化甲基化DNA所需的酶量取决于SgeI识别位点的数量。由靶位点切割产生的DNA切割产物促进了SgeI的非特异性切割。因此，建议优化酶量以进行DNA切割。从大肠杆菌dcm +菌株（＃SD0041）分离的pBR322 DNA可用作DNA切割效率对照。 SgeI切割pBR322 DNA上所有六个dcm甲基化的靶标。有关甲基化敏感性信息，请参阅产品说明。

优点：

• Superior quality—stringent quality control and industry leading manufacturing process• Convenient color-coded Five Buffer System• Includes universal Tango buffer for double-digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificities• 优异的质量—严格的质量控制和业内领先的生产流程• 使用颜色标记的五大便捷缓冲系统• 包含适于双酶切的通用型Tango缓冲液• 反应缓冲液中预先混入BSA• 种类齐全，特异性限制性内切酶的广泛选择

数据：

技术参数

产品优点 • Superior quality—stringent quality control and industry leading manufacturing process • Convenient color-coded Five Buffer System • Includes universal Tango buffer for double-digestions • BSA premixed in reaction buffers • Wide selection of restriction endonuclease specificities • 优异的质量—严格的质量控制和业内领先的生产流程 • 使用颜色标记的五大便捷缓冲系统 • 包含适于双酶切的通用型Tango缓冲液 • 反应缓冲液中预先混入BSA • 种类齐全，特异性限制性内切酶的广泛选择

产品应用 • Molecular cloning • Restriction site mapping • Genotyping • Southern blotting • Restriction fragment length polymorphism (RFLP) • SNP •分子克隆 •限制性位点比对 •基因分型 •Southern印迹 •限制性片段长度多态性（RFLP） •SNP（单核苷酸多态性）

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DNA内切酶，thermo,SgeI (3 U/µL)/SgeI内切酶

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