DNA内切酶 Thermo FastDigest HindIII 快酶HindIII FD0504,FastDigest HindIII/快酶HindIII-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

DNA内切酶 Thermo FastDigest HindIII 快酶HindIII FD0504,FastDigest HindIII/快酶HindIII

2024-10-24

FastDigest HindIII/快酶HindIII

货号：FD0504,FD0505

规格：800 reactions,2500 reactions

价格：455,1427

产品类型：内切酶

品牌：Thermo Fisher

Thermo Scientific FastDigest HindIII restriction enzyme recognizes A^AGCTT site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer.Thermo Scientific FastDigest HindIII is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. SeeReaction Conditions for FastDigest Enzymesfor a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such asKlenow Fragment,T4 DNA Ligase, alkaline phosphatases andT4 DNA Polymeraseall have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.Note:The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.Thermo Scientific FastDigest HindIII限制性内切酶可识别A ^ AGCTT位点，并使用通用FastDigest缓冲液在37°C下5-15分钟内最佳切割。Thermo Scientific FastDigest HindIII是优异的快速限制酶系列之一，在通用FastDigest和FastDigest绿色反应缓冲液中均具有100％的活性。通用缓冲液允许5-15分钟内快速进行单，双或多个DNA消化，从而无需更换缓冲液或随后的DNA净化步骤。有关此酶和其他FastDigest限制酶的酶活性，热灭活和孵育时间，请参见FastDigest 酶的反应条件。DNA 修饰酶（如Klenow 片段、T4 DNA 连接酶、碱性磷酸酶和T4 DNA 聚合酶）在 FastDigest 缓冲液中均具有 100% 活性。因此，可将用于下游应用的酶直接添加到FastDigest反应混合物中。为了方便进一步使用，FastDigest 绿色缓冲液包括一种密度试剂和两种跟踪染料，可用于将消化反应产物直接加载到凝胶上。较短的孵育时间和通用FastDigest缓冲液的最佳组合消除了星号活性的影响。注意：FastDigest绿色缓冲液在DNA消化和下游应用中提供与无色FastDigest缓冲液相同的高性能。对于需要通过荧光激发进行产品分析的应用（例如，紫外光中的浓度测量），建议使用无色的FastDigest缓冲液。对 dam 甲基化不敏感、对 dcm 甲基化不敏感、对 CpG 甲基化不敏感.

优点：

- 100% activity of all FastDigest enzymes in the universal buffer- 100% buffer compatibility with downstream applications- Complete digestion in 5–15 minutes- Direct loading on gels- No star activity- 176 FastDigest enzymes available- 所有 FastDigest 酶在通用型缓冲液中的 100% 活性- 与下游应用的 100% 缓冲液兼容性- 5–15 分钟内即可完成酶切- 直接上样至凝胶- 无星号活性- 可提供 176 种 FastDigest 酶

数据：

技术参数

产品优点 1. 所有 FastDigest 酶在通用型缓冲液中的 100% 活性 2. 与下游应用的 100% 缓冲液兼容性 3. 5–15 分钟内即可完成酶切 4. 直接上样至凝胶 5. 无星号活性 6. 可提供 176 种 FastDigest 酶

产品应用 •分子克隆 •限制位点作图 •基因分型 •Southern 印迹 •限制性片段长度多态性 (RFLP) •单核苷酸多态性（SNP）

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DNA内切酶 Thermo FastDigest HindIII 快酶HindIII FD0504,FastDigest HindIII/快酶HindIII

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