ESRRB Recombinant Rabbit Monoclonal Antibody (9H8L3)

货号：703237

规格：100 µg

价格：4574

产品类型：抗体和染料

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号： 9H8L3

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	1:100 (ICC/IF)

产品详细信息This antibody is predicted to react with Monkey, Horse, Pig, Cat.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息ESRRB (steroid hormone receptor ERR2) plays a role in maintenance of self-renewal and pluripotency of embryonic and trophoblast stem cells through different signaling pathways including FGF signaling pathway and Wnt signaling pathways. Upon FGF signaling pathway activation ESRRB interacts with KDM1A by directly binding to enhancer sites of ELF5 and EOMES and activates their transcription leading to self-renewal of trophoblast stem cells. ESSRB also regulates expression of multiple rod-specific genes and is required for survival of this cell type. Defects in the gene can cause deafness, autosomal recessive, 35 (DFNB35).

数据

ESRRB Antibody (703237) in ICC/IFFor immunofluorescence analysis, iPS cells were fixed and permeabilized for detection of endogenous ESRRB using Anti-ESRRB Recombinant Rabbit Monoclonal Antibody (Product # 703237, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) Panel a-d) shows representative control cells that were stained for detection and localization of ESRRB protein (green) with enhanced nuclear signal compared to panel e-h) showing no signal in cells differentiated to neuronal phenotype demonstarting specificity. The images were captured at 60X magnification.

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