DNA内切酶 Thermo 快酶NdeI FD0583 ,FastDigest NdeI (FauNDI)/快酶NdeI

2024-10-24

FastDigest NdeI (FauNDI)/快酶NdeI

货号:FD0583,FD0584,FD0585

规格:100 reactions,300 reactions,1000 reactions

价格:329,594,1760

产品类型:内切酶

品牌:Thermo Fisher

Thermo Scientific FastDigest NdeI restriction enzyme recognizes CA^TATG site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: FauNDI.Thermo Scientific FastDigest NdeI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. SeeReaction Conditions for FastDigest Enzymesfor a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such asKlenow Fragment,T4 DNA Ligase, alkaline phosphatases andT4 DNA Polymeraseall have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.Note:The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.Thermo Scientific FastDigest NdeI 限制性内切酶可识别 CA^TATG 位点,使用通用型 FastDigest 缓冲液于 37°C 下在5–15 分钟内的切割效果最佳。同裂酶:FauNDI。Thermo Scientific FastDigest NdeI 是一系列快速限制性内切酶之一,该系列在通用型 FastDigest 和 FastDigest 绿色反应缓冲液中均具备 100% 活性。通用型缓冲液支持在 5–15 分钟内快速完成 DNA 单酶切、双酶切或多酶切,无需更换缓冲液或后续 DNA 纯化步骤。有关该酶和其他 FastDigest 限制性内切酶的酶活性、热灭活和孵育时间的表格,请参见FastDigest 酶的反应条件。DNA 修饰酶(如Klenow 片段、T4 DNA 连接酶、碱性磷酸酶和T4 DNA 聚合酶)在 FastDigest 缓冲液中均具有 100% 活性。因此,在下游应用中使用的酶可直接添加至 FastDigest 反应混合物中。为了方便起见,FastDigest 绿色缓冲液包括用于直接将酶切反应产品上样至凝胶的 1 种密度试剂和 2 种示踪染料。较短的孵育时间和较优的通用型 FastDigest 缓冲液组成消除了星活性效应。备注:FastDigest 绿色缓冲液在 DNA 酶切反应和下游应用中具有和无色 FastDigest 缓冲液一样的高性能。对于需要通过荧光激发进行产物分析的应用(例如在紫外光下测定浓度),则建议使用无色 FastDigest 缓冲液。对 dam 甲基化不敏感、对 dcm 甲基化不敏感、对 CpG 甲基化不敏感。
优点:
- 100% activity of all FastDigest enzymes in the universal buffer- 100% buffer compatibility with downstream applications- Complete digestion in 5–15 minutes- Direct loading on gels- No star activity- 176 FastDigest enzymes available- 所有 FastDigest 酶在通用型缓冲液中的 100% 活性- 与下游应用的 100% 缓冲液兼容性- 5–15 分钟内即可完成酶切- 直接上样至凝胶- 无星号活性- 可提供 176 种 FastDigest 酶
数据:

技术参数

产品优点 1. 所有 FastDigest 酶在通用型缓冲液中的 100% 活性 2. 与下游应用的 100% 缓冲液兼容性 3. 5–15 分钟内即可完成酶切 4. 直接上样至凝胶 5. 无星号活性 6. 可提供 176 种 FastDigest 酶

产品应用 •分子克隆 •限制位点作图 •基因分型 •Southern 印迹 •限制性片段长度多态性 (RFLP) •单核苷酸多态性(SNP)

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