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F564L PCR Thermo ,Phusion U Green Multiplex PCR Master Mix/绿色多重PCR混合液

2024-10-24

Phusion U Green Multiplex PCR Master Mix/绿色多重PCR混合液

货号：F564L,F564S

规格：500 reactions,100 reactions

价格：7035,1788

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

Thermo Scientific Phusion U Green Multiplex PCR Master Mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube. Over 20 primer pairs may be combined into a single reaction for highly specific and efficient multiplexing over a broad range of primer and template concentrations.The Phusion U Green Multiplex PCR Master Mix contains a proprietary buffer with balanced concentrations of all PCR components eliminating the need for tedious optimization. The Green format further simplifies the workflow - it includes a density reagent and two electrophoresis tracking dyes for direct loading of PCR products on gels.The master mix is based on Phusion U Hot Start DNA Polymerase, a high performance enzyme developed using fusion technology. Similarly to other enzymes from Phusion family, Phusion U DNA Polymerase incorporates more nucleotides per binding event than any other DNA polymerase. This allows achieving high yields of PCR products with shorter extention times and consequently reduced total protocol times. Significantly enhanced processivity also enables successful multiplex PCR on difficult targets such as GC-rich templates or templates of suboptimal purity. Being highly tolerant of many PCR inhibitors, Phusion U Multiplex PCR Master Mixes can even be used with unpurified samples such as blood or serum.Specificity of multiplex PCR is increased by Affibody-mediated hot start mechanism. Reversibly bound Affibody ligand inhibits the polymerase at ambient temperatures preventing amplification of nonspecific products and formation of primer-dimers.

优点：

- Specific and efficient multiplex PCR of over 20 targets up to 2.5 kb- Excellent results with templates of suboptimal purity due to inhibitor tolerance- Fast cycling and direct loading of PCR products on gels

数据：

图1.人Efficient multiplex PCR in short reaction times19 fragments (73-2527 bp) from human genomic DNA were simultaneously amplified using Phusion U Green Multiplex PCR Master Mix and multiplex PCR master mixes from other suppliers. All reactions were performed according to manufacturers’ recommendations. Only Phusion U Green Multiplex Master Mix delivered specific amplification of all 19 fragments. Furthermore, high processivity of Phusion U DNA polymerase enabled short extension times and resulted in the shortest PCR protocol.M - GeneRuler 100bp Plus DNA Ladder

图2.Multiplex PCR using whole blood samples14 fragments (70-1000 bp) from human genomic DNA were simultaneously amplified from human blood with Phusion U Green Multiplex PCR Master Mix and multiplex PCR master mixes from other suppliers. 2 µL of whole blood was added directly to 50 µL final reaction volume and cycling was performed according to manufacturers’ recommendations. Enhanced processivity of Phusion U DNA Polymerase makes it highly tolerant to many common PCR inhibitors including those from blood samples. Consequently, only Phusion U Green Multiplex PCR Master Mix was able to amplify multiple fragments directly from blood.M - GeneRuler 100bp Plus DNA Ladder

组成成分：

▪Phusion U Hot Start DNA Polymerase▪ 2X reaction buffer▪ 3 mM MgCl2▪ 400 µM of each dATP, dTTP, dCTP and dGTPThe master mix is supplied with nuclease-free water.Store at -20°C.

F564L PCR Thermo ,Phusion U Green Multiplex PCR Master Mix/绿色多重PCR混合液

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