2. 试剂
  3. F556L DNA聚合酶 Thermo ,Phusion U Green Hot Start DNA Polymerase/绿色热启动DNA聚合酶

F556L DNA聚合酶 Thermo ,Phusion U Green Hot Start DNA Polymerase/绿色热启动DNA聚合酶

2024-10-24
Phusion U Green Hot Start DNA Polymerase/绿色热启动DNA聚合酶

货号：F556L,F556S

规格：500 units,100 units

价格：5064,1276

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes - it is able to incorporate dUTP and read through uracil present in DNA templates.In addition to uracil usage possibility, Phusion U features all the superior properties of other Phusion DNA Polymerases - great accuracy, speed, ability to amplify long amplicons up to 20 kb and a high specificity with Affibody based hot start. These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control.Phusion U Hot Start Green DNA Polymerase is a combination of Phusion U Hot Start DNA Polymerase and 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with Phusion U performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

优点：

-Accuracy—high fidelity DNA polymerase (25x Taq)-Uracil-tolerance—engineered to incorporate dUTP and amplify uracil containing templates-Specificity—hot start for reduced nonspecific amplification and primer degradation-Speed—short extension times (15-30 s/kb)-Green format—permits direct loading of PCR products on gels

组成成分：

▪Phusion U Hot Start DNA Polymerase, 2 U/µL▪ 5X Phusion Green HF Buffer▪ 5X Phusion Green GC Buffer▪ DMSOBoth Phusion Green HF Buffer and Phusion Green GC Buffer provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.

相关产品：

▪DNA 片段标准品▪DNA ladder▪DNA 聚合酶▪PCR▪DNA纯化▪RNA纯化

技术参数

产品应用 • Amplification of bisulphite-converted DNA • Amplification of damaged or aged DNA • Carry-over contamination control • Uracil-excision based (USER) cloning methods

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