F562L Thermo PCR,Phusion U Multiplex PCR Master Mix/多色 PCR预混液-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

F562L Thermo PCR,Phusion U Multiplex PCR Master Mix/多色 PCR预混液

2024-10-24

Phusion U Multiplex PCR Master Mix/多色 PCR预混液

货号：F562L,F562S

规格：500 reactions,100 reactions

价格：7035,1788

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

Thermo Scientific Phusion U Multiplex PCR Master Mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube. Over 20 primer pairs may be combined into a single reaction for highly specific and efficient multiplexing over a broad range of primer and template concentrations.The Phusion U Multiplex PCR Master Mix contains a proprietary buffer with balanced concentrations of all PCR components eliminating the need for tedious optimization. The Phusion U Green Multiplex PCR Master Mix (F-564S or F-564L) further simplifies the workflow - it includes a density reagent and two electrophoresis tracking dyes for direct loading of PCR products on gels.The master mix is based on Phusion U Hot Start DNA Polymerase, a high performance enzyme developed using fusion technology. Similarly to other enzymes from Phusion family, Phusion U DNA Polymerase incorporates more nucleotides per binding event than any other DNA polymerase. This allows achieving high yields of PCR products with shorter extention times and consequently reduced total protocol times. Significantly enhanced processivity also enables successful multiplex PCR on difficult targets such as GC-rich templates or templates of suboptimal purity. Being highly tolerant of many PCR inhibitors, Phusion U Multiplex PCR Master Mix can even be used with unpurified samples such as blood or serum.Specificity of multiplex PCR is increased by Affibody-mediated hot start mechanism. Reversibly bound Affibody ligand inhibits the polymerase at ambient temperatures preventing amplification of nonspecific products and formation of primer-dimers.

优点：

- Specific and efficient multiplex PCR of over 20 targets up to 2.5 kb- Excellent results with templates of suboptimal purity due to inhibitor tolerance- Fast cycling and direct loading of PCR products on gels with Green format (F-564S and F-564L)

数据：

图1.Robust multiplex PCR even with limited amount of DNA templateSensitivity of several multiplex PCR master mixes was tested by amplifying 14 targets (70-1000 bp) from decreasing amounts of human genomic DNA. Only Phusion U Multiplex PCR master Mix was able to efficiently amplify all targets even from the smallest genomic DNA input (0.1 ng).M - GeneRuler 100bp DNA Ladder

图2.Highly multiplex PCR: simultaneous amplification of over 20 targets up to 2.5 kb20 ng of human genomic DNA was used as a template for simultaneous amplification of 5, 10, 15, 20 and 24 fragments using Phusion U Multiplex PCR Master Mix according to manufacturer’s recommendations. Size range of amplicons was from 73 bp to 2.5 kb, and GC content varied in a range of 35-67%.M1 - GeneRuler 50bp DNA LadderM2 -GeneRuler 100bp Plus DNA Ladder

组成成分：

▪Phusion U Hot Start DNA Polymerase▪ 2X reaction buffer▪ 3 mM MgCl2▪400 µM of each dATP, dTTP, dCTP and dGTPThe master mix is supplied with nuclease-free water.Store at -20°C.

F562L Thermo PCR,Phusion U Multiplex PCR Master Mix/多色 PCR预混液

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