F533L,Phusion U Hot Start PCR Master Mix/Phusion U热启动 PCR混合物-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

F533L,Phusion U Hot Start PCR Master Mix/Phusion U热启动 PCR混合物

2024-10-24

Phusion U Hot Start PCR Master Mix/Phusion U热启动 PCR混合物

货号：F533L,F533S

规格：500 reactions,100 reactions

价格：7202,1818

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology. Due to a proprietary mutation in the so called dUTP binding pocket of Phusion, Phusion U overcomes an important limitation of proofreading enzymes - it is able to incorporate dUTP and read through uracil present in DNA templates.In addition to uracil usage possibility, Phusion U features all the superior properties of other Phusion DNA Polymerases - great accuracy, speed, ability to amplify long amplicons up to 20 kb and a high specificity with Affibody based hot start. These features make Phusion U Hot Start DNA Polymerase an excellent choice for such important applications as amplification of bisulphite-converted or damaged DNA as well as use of carryover contamination control.Phusion U Hot Start PCR Master Mix is convenient 2X mix containing Phusion U Hot Start DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added by the user.

优点：

-Accuracy—high fidelity DNA polymerase (25x Taq)-Uracil-tolerance—engineered to incorporate dUTP and amplify uracil containing templates-Specificity—hot start for reduced nonspecific amplification and primer degradation-Speed—short extension times (15-30 s/kb)

数据：

图1.Incorporates dUTP into PCR products2 kb fragment from 50 ng human genomic DNA amplified in the presence of 0.2 mM dUTP according to recommendations of different suppliers of proofreaders with dUTP incorporation ability.

图2.Highest yields and specificity with bisulphite-converted DNAFive proofreading DNA polymerases and hot startTaqpolymerase were used to amplify 798 bp fragment of bisulphite-converted human genomic DNA. Phusion U Hot Start DNA Polymerase provided high yields of specific products whereas all other enzymes delivered zero or lower yields, with some also amplifying non-specific products.

组成成分：

▪Phusion U Hot Start DNA Polymerase▪ 2X reaction buffer▪ 3 mM MgCl2▪ 400 µM of each dATP, dTTP, dCTP and dGTPThe master mix is supplied with nuclease-free water.Store at -20°C.

F533L,Phusion U Hot Start PCR Master Mix/Phusion U热启动 PCR混合物

相关推荐