F530L DNA聚合酶 Thermo ,Phusion™ High-Fidelity DNA Polymerase (2 U/µL)/Phusion™ 高保真DNA聚合酶 (2 U/µL)-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

F530L DNA聚合酶 Thermo ,Phusion™ High-Fidelity DNA Polymerase (2 U/µL)/Phusion™ 高保真DNA聚合酶 (2 U/µL)

2024-10-24

Phusion™ High-Fidelity DNA Polymerase (2 U/µL)/Phusion™ 高保真DNA聚合酶 (2 U/µL)

货号：F530L,F-530XL,F530S

规格：500 units,2000 units,100 units

价格：3105,9918,783

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

Thermo Scientific Phusion 高保真 DNA 聚合酶为高效 PCR 设立了“金标准”。Phusion 高保真 DNA 聚合酶的错误率是Taq的 1/50，是Pfu的 1/6，因此是克隆和其它需要高保真度应用的极佳之选。与其它 DNA 聚合酶相比，Phusion DNA 聚合酶性能稳定，实验方案耗时少（即使存在 PCR 抑制剂），能以更低的酶量实现更高的产出。应用• 高保真 PCR• 克隆• 获得测序模板• 困难（高 GC 含量）模板扩增• 长片段 PCR（达 20 kb）• 突变• 高通量 PCR• 微阵列

优点：

- 高保真度 (52XTaq)- 延伸时间短 (15-30 s/kb)，可实现快速 PCR- 反应稳健，几乎无需优化- 能以尽可能少的酶用量实现产物高得率- 有 Green 缓冲液规格可供选择，用于将 PCR 产物直接上样至凝胶（F-534S 或 F-534L）

数据：

图1.Superior yields of long PCR products20kb fragment from λ DNA and 7.5 kb fragment from human genomic DNA was amplified with Phusion and proofreading DNA polymerase from other suppliers. Phusion and Phusion Green DNA Polymerases were the only enzymes capable of providing high amounts of desired PCR products. In contrast, other proofreading polymerases delivered zero or significantly lower yields.

图2.Phusion DNA Polymerase combines extreme fidelity with unparalleled speed and robustnessA random set of 16 clones from aThermus sp.genomic library was amplified from bacterial colonies. The results highlight the robustness and speed of Phusion DNA Polymerase. It was able to amplify 94% of the amplicons with lower enzyme amounts still producing superior yields. The success rate ofPfuDNA polymerase was only 56% and that ofTaqDNA polymerase 62% with significantly lower yields.

图3.Extreme fidelity for demanding PCRRelative fidelity values of different DNA polymerases. Phusion DNA Polymerases have extremely low error rates. The error rate, determined by a modified lacI-based method, is approximately 50-fold lower than that ofTaqDNA polymerase and 6-fold lower than that ofPfuDNA polymerase. Fidelity = 1/error rate.

图4.Less enzyme - superior yieldA 3.8kb fragment from human beta globin gene was amplified with three different DNA polymerases. Phusion DNA Polymerase was able to amplify the 3.8kb genomic fragment with a combined annealing and extension step of only 1minute, thus being significantly faster than the two other polymerases tested. A single unit of Phusion DNA Polymerase produced higher yields than 2.5 or 5 units of the Pfu DNA polymerases.

组成成分：

- Phusion DNA 聚合酶 (2 U/µL)- 5X Phusion HF 缓冲液- 5X Phusion GC 缓冲液- DMSO- 50 mM MgCl₂溶液Phusion HF 缓冲液和 Phusion GC 缓冲液都提供最终 1X 浓度的 1.5 mM MgCl₂。在-20°C 条件下储存。

F530L DNA聚合酶 Thermo ,Phusion™ High-Fidelity DNA Polymerase (2 U/µL)/Phusion™ 高保真DNA聚合酶 (2 U/µL)

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