T4 beta-glucosyltransferase/T4 β-葡糖基转移酶

货号：EO0831

规格：500 U

价格：1680

产品类型：酶及抑制剂

品牌：Thermo Fisher

描述Thermo Scientific T4 β-glucosyltransferase (T4 BGT) transfers the glucose moiety of uridine diphosphoglucose (UDP-glucose) to the 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA generating ß-glucosyl-5 hydroxymethylcytosine.Thermo Scientific T4 BGT is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes 5-hmC glucosylation of 1 µg DNA at 37°C in 15 minutes.Highlights•Specific—selectively transfers glucose to the hydroxymethyl moiety of 5-hmC•Fast—complete glucosylation of 1 µg DNA in just 15 min•Convenient—supplied with optimized buffer and UDP-glucose.Applications• Locus specific detection of 5-hmC• Enrichment of 5-hmC containing DNA• Labeling of 5-hmC residues using a radioactive UDP-glucose donorIncludes• T4 β-glucosyltransferase, 5 U/µL• 10X Epi buffer• 10X UDP-glucoseSuperior glucosylation in 15 minutes1 µg of a fully 5-hydroxymethylated 1095 bp PCR fragment was incubated with 1 µL of Thermo Scientific T4 BGT and compared to the performance of T4 BGT from other suppliers (1-3) following recommended protocols. Reaction products were digested with the glucosyl-sensitive restriction enzyme MunI and analyzed by electrophoresis on a 1% agarose gel.

技术参数

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