K0513 DNA提取 Thermo ,Silica Bead DNA Gel Extraction Kit/二氧化硅珠DNA凝胶提取试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

K0513 DNA提取 Thermo ,Silica Bead DNA Gel Extraction Kit/二氧化硅珠DNA凝胶提取试剂盒

2024-10-24

Silica Bead DNA Gel Extraction Kit/二氧化硅珠DNA凝胶提取试剂盒

货号：K0513

规格：100 preps

价格：516

产品类型：DNA提取

品牌：Thermo Fisher

Thermo Scientific Silica Bead DNA Gel Extraction Kit is a simple and efficient system for DNA extraction from agarose gels and reaction mixtures. The kit utilizes the modified protocol of Vogelstein and Gillespie, employing solubilization of the agarose gel and selective adsorption of nucleic acids on specially prepared silica particles at high salt concentration. Silica particles bound with DNA are washed to remove contaminants and pure DNA is eluted with TE buffer or water.This method requires few manipulations, and is both faster and easier to perform than other organic-based extraction methods. Compared to column based gel-extraction kits, the DNA Gel Extraction Kit offers flexibility for scaling reaction and elution volumes up or down. The purified DNA is suitable for all common molecular biology procedures, including restriction digestion, cloning, sequencing, etc.Includes Silica Powder Suspension Binding Buffer Concentrated Washing Buffer TBE Conversion Buffer Detailed ProtocolNoteCare should be taken to avoid DNA damage with UV light when the gel-purified DNA fragment will be used in downstream cloning reactions. Always use a long wavelength UV (360 nm) light-box during excision of the gel slice. If only a short-wavelength UV (254 to 312 nm) light-box is available, minimize the UV exposure to a few seconds or keep the gel on a glass or plastic plate. Alternatively, visible dyes can be included in standard agarose gels to allow for visualization of the DNA bands in ambient light (2, 3).

优点：

-Flexible—easy to scale up or down both reaction and elution volumes-Efficient—greater than 80% DNA recovery-Fast—purification takes only 15 to 20 minutes-Effective—suitable for DNA fragments as short as 100 bp-Safe—does not involve phenol extraction

数据：

图1.Recovery of 100 to 3000bp DNA fragments using the Silica Bead DNA Gel Extraction KitRecovery of GeneRuler 100bp Plus DNA Ladder individual 100 to 3000bp DNA fragments from a 1.7% agarose gel using the Silica Bead DNA Gel Extraction Kit.M – GeneRuler 100 bp Plus DNA Ladder.

图2.从琼脂糖凝胶中提取DNA

组成成分：

▪Silica Powder Suspension (glass bead suspension in water)▪Binding Buffer▪Concentrated Washing Buffer▪TBE Conversion Buffer

K0513 DNA提取 Thermo ,Silica Bead DNA Gel Extraction Kit/二氧化硅珠DNA凝胶提取试剂盒

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