DreamTaq Green DNA Polymerase (5 U/µL)聚合酶

货号：EP0711,EP0712,EP0713,EP0714

规格：200 units,500 units,5 x 500 units,20 x 500 units

价格：119,270,1103,3117

产品类型：PCR 及DNA聚合酶

品牌：Thermo Fisher

描述Thermo Scientific DreamTaq Green DNA Polymerase is a combination of DreamTaq DNA Polymerase and 10X DreamTaq Green Buffer. DreamTaq DNA Polymerase is an enhancedTaqpolymerase optimized for high throughput PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventionalTaqDNA polymerase. Extensive optimization of reaction conditions is not required.The 10X DreamTaq Green Buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with PCR performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion. For applications requiring PCR product analysis by absorbance or fluorescence excitation, we recommend using the colorless 10X DreamTaq Buffer (#B65) or purifying the PCR product before analysis. The 10X DreamTaq Green Buffer is optimized for robust performance in PCR and includes MgCl2at a concentration of 20mM.Highlights• Direct loading of PCR product on gel• High yields and high sensitivity of PCR• Amplification of long targets up to 6kb from genomic DNA and up to 20kb from viral DNA• Robust amplification with minimal optimization• Incorporates modified nucleotides, but does not incorporatedUTPApplications• Routine PCR amplification of DNA fragments up to 6kb• Genotyping• High throughput PCRLonger PCR fragments, higher yields with DreamTaq Green DNA PolymeraseAmplification of PCR fragments from complex mouse and human genomic DNA using DreamTaq Green DNA Polymerase and other commercial enzymes that allow direct gel loading of PCR mixtures. PCR was performed according to supplier recommendations in a 50µL reaction volume and 10µL of each PCR mixture was loaded on the gel.M – GeneRuler Express DNA Ladder1 – 424bplcsgene fragment from mouse genomic DNA2 – 956bp 7 chromosome STS (G31656) from human genomic DNA3 – 2537bptPAgene fragment from human genomic DNA4 – 5003bp beta-globine gene fragment from human genomic DNA

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