K0761 RNA纯化 Thermo ,GeneJET Whole Blood RNA Purification Mini Kit/GeneJET全血RNA纯化试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

K0761 RNA纯化 Thermo ,GeneJET Whole Blood RNA Purification Mini Kit/GeneJET全血RNA纯化试剂盒

2024-10-24

GeneJET Whole Blood RNA Purification Mini Kit/GeneJET全血RNA纯化试剂盒

货号：K0761

规格：50 preps

价格：1994

产品类型：RNA提取

品牌：Thermo Fisher

Thermo Scientific GeneJET全血RNA小量纯化试剂盒专为从全血及相关体液样本中快速高效地纯化高质量总RNA而设计。该试剂盒使用独有的基于硅膜吸附技术的离心柱，无需昂贵的树脂纯化、有毒的苯酚/氯仿提取或耗时的乙醇沉淀。细胞裂解后的标准实验流程不超过15 分钟。纯化的高质量RNA 可用于各类下游实验，例如RT-PCR、RT-qPCR，Northern blotting和其它基于RNA的分析实验。

优点：

-高效– 从500µL人类血液样本中可纯化多达1.8 µg的总RNA-便捷– 离心柱加盖并预装入收集管内，单独包装-纯度高– 分离出的RNA不含酶抑制剂和蛋白，可立即用于各种高标准实验，在cDNA合成和RT-qPCR中均进行过验证-简单、快速– 流程所需时间少于15分钟，不含有机抽提或血红细胞（RBC）裂解-兼容性强– 适用于各类样本，例如人或其他哺乳动物的血液（经EDTA、柠檬酸或肝素处理）、血液棕黄层、骨髓和其它体液等

数据：

图1.Stabilization of blood samples by Lysis BufferRNA purified from blood samples stored at various temperatures with Lysis Buffer. 500µL of whole blood centrifuged, cells resuspended in 600µL of Lysis Buffer and either used immediately (lanes 1 to 4) or stored for 24h at 4°C (lanes 5 to 8), or for 7 days at -20°C (lanes 9 to 12) before processing with GeneJET Whole Blood RNA Purification Mini Kit. RNA integrity analyzed using Agilent 2100 Bioanalyzer.1 to 4– RNA from fresh blood5-8– RNA from samples stored in Lysis Buffer for 24 h at +4°C9-12– RNA from samples stored in Lysis Buffer for 7 days at -20°C

图2.Total RNA purified from human blood using the GeneJET Whole Blood RNA Purification Mini Kit5 µL (10%) of RNA isolated from 500µL of human blood was fractionated by agarose gel (1%) electrophoresis.1– Thermo Scientific2 to 4– Various vendors

图3.纯化流程

组成成分：

▪Lysis Buffer▪Wash Buffer WB 1 (concentrated)▪Wash Buffer 2 (concentrated)▪Water, nuclease-free▪GeneJET RNA Purification Columns pre-assembled with Collection Tubes▪Collection Tubes, 2 mL▪Collection Tubes, 1.5 mL

K0761 RNA纯化 Thermo ,GeneJET Whole Blood RNA Purification Mini Kit/GeneJET全血RNA纯化试剂盒

相关推荐