DNA纯化 K0781 Thermo ,GeneJET Whole Blood Genomic DNA Purification Mini Kit/GeneJET全血基因组DNA纯化试剂盒-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

DNA纯化 K0781 Thermo ,GeneJET Whole Blood Genomic DNA Purification Mini Kit/GeneJET全血基因组DNA纯化试剂盒

2024-10-24

GeneJET Whole Blood Genomic DNA Purification Mini Kit/GeneJET全血基因组DNA纯化试剂盒

货号：K0781,K0782

规格：50 preps,250 preps

价格：965,4330

产品类型：DNA提取

品牌：Thermo Fisher

Thermo Scientific GeneJET Whole Blood Genomic DNA Purification Mini Kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions.

优点：

-Efficient—high yields of high molecular weight genomic DNA-Fast—20 minute procedure following cell lysis-Convenient—spin columns are capped and assembled with collection tubes-Pure—DNA pure from enzyme inhibitors, RNA and proteins. Ready to use for all demanding applications and validated in real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests-Compatible—suitable for wide variety of samples like fresh or frozen anticoagulated mammalian blood (treated with EDTA, Citrate or Heparine), aged blood, buffy coat, bone marrow, and body fluids

数据：

图1.Accurate evaluation of genomic DNA yieldsGenomic DNA was purified from human blood using the GeneJET Whole Blood Genomic DNA Purification Mini Kit and kits from other vendors. DNA concentration was estimated using Nanodrop spectrophotometer (Thermo Scientific) and 200pg used as template in qPCR with primers against single copy gene. Matching yields obtained by qPCR based absolute quantification and spectrophotometrical evaluation indicate absence of reaction inhibitors and contaminating RNA.

图2.Genomic DNA purified from various sources using the GeneJET Whole Blood Genomic DNA Purification Mini Kit200ng of isolated genomic DNA from each source was fractionated by agarose gel (0.5%) electrophoresis.M – Lambda DNA/HindIII Marker (#SM0101)1 – human whole blood2 – human buffy coat3 – human bone marrow4 – avian whole blood. 200ng of isolated genomic DNA from each source was fractionated by agarose gel (0.5%) electrophoresisM – Lambda DNA/HindIII Marker (#SM0101)1 – human whole blood2 – human buffy coat3 – human bone marrow4 – avian whole blood

图3.纯化流程

组成成分：

▪Proteinase K Solution▪Lysis Solution▪Wash Buffer WB I (concentrated)▪Wash Buffer II (concentrated)▪Elution Buffer (10 mM Tris-HCl, pH 9.0, 0.1 mM EDTA)▪GeneJET Genomic DNA Purification Columns pre-assembled with Collection Tubes▪Collection Tubes (2 mL)

DNA纯化 K0781 Thermo ,GeneJET Whole Blood Genomic DNA Purification Mini Kit/GeneJET全血基因组DNA纯化试剂盒

相关推荐