Rapid DNA Ligation Kit

货号：K1422,K1423

规格：50 reactions,150 reactions

价格：1912,3601

产品类型：分子生物学

品牌：Thermo Fisher

描述Thermo Scientific Rapid DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.The kit contains T4 DNA ligase and a specially-formulated 5X rapid ligation buffer optimized for fast and efficient DNA ligation. Fast ligation efficiency is equal to that obtained with T4 DNA ligase in a standard 1 hour ligation. The ligation reaction mixture can be used directly for bacterial transformation with theTransformAid Bacterial Transformation Kitor with other conventional transformation procedures.Highlights• Fast—ligation of either sticky- or blunt-end DNA in only 5 minutes• Convenient—reaction mixture can be used directly for bacterial transformationApplications• Routine cloning experiments• Blunt-end cloning• Library construction• TA cloningIncludes•T4 DNA Ligase• 5X Rapid Ligation Buffer•Water, nuclease-free• Detailed ProtocolNotePrior to electroporation, it is necessary to inactivate T4 DNA ligase by chloroform extraction or spin column purification, e.g. withThermo Scientific GeneJET PCR Purification Kit(K0701).Related ProductsRapid DNA Ligation KitLigation of sticky-, blunt-end DNA fragments with the Rapid DNA Ligation KitBlunt-end ligation. pUC19 DNA/SmaI dephosphorylated vector was ligated with Lambda DNA/PvuII fragment (2.3kb). Sticky-end ligation. pUC19 DNA/PstI dephosphorylated vector was ligated with Lambda DNA/PstI fragment (2.1kb). The efficiency of ligation was determined by transformation ofE. coliXL1-Blue cells.

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