MuSeek Library Preparation Kit, Illumina compatible

货号：K1361

规格：12 reactions

价格：1

产品类型：分子生物学

品牌：Thermo Fisher

描述Thermo Scientific™ MuSeek™ Library Preparation Kit, Illumina™ compatible is designed for generation of high‐quality genomic DNA libraries for sequencing with the Illumina™ MiSeq™ and HiSeq™ systems. The fast protocol utilizes the MuA transposase enzyme, which catalyzes simultaneous fragmentation of double-stranded target DNA and tagging the fragment ends with transposon DNA. In a subsequent PCR step the platform-specific adapters are added using a robust and accurate Thermo Scientific Phusion™ High-Fidelity DNA polymerase. Starting with only 50 ng or 100 ng of sample the protocol can be used to generate 100 bp - 1,000 bp insert libraries. The kit contains components sufficient for 12 fragmentation reactions and the subsequent adapter-addition polymerase chain reactions. Sequencing primers required for sequencing libraries prepared by this method are provided as well.Highlights•Convenient—no physical shearing, end-repair and ligation steps•Fast—generates NGS fragment libraries in less than 90 minutes•Low sample input—DNA fragment libraries from 50 ng DNA input•Indexing compatible—MuSeek Index Set 1 enables preparation of up to 24 dual-indexed DNA libraries for sequencing in one run.Applications• DNA fragment library preparation for downstream use in next generation sequencing workflow.• Library preparation from gDNA, ds DNA, bacterial DNA, DNA amplicons.Includes• MuSeek Enzyme Mix, IL• MuSeek Fragmentation Reaction Buffer• MuSeek Stop Solution• Water, nuclease-free• Control DNA• MuSeek Adapter Addition Primer Mix, IL• MuSeek Adapter Addition Reaction Buffer, IL• Phusion™ Hot Start II High-Fidelity DNA Polymerase, IL• MuSeek Sequencing Primer, Read 1• MuSeek Sequencing Primer, Read 2• MuSeek Sequencing Primer, Index ReadNotesThe kit is shipped on dry ice. Upon arrival store at -20°C, avoid repeated freeze-thaw cycles. The Stop Solution can be stored at room temperature.Exact library length size selectionFlexible DNA fragment library lengthRelative size distribution ofE. coligenomic DNA fragment library generated using the MuSeek Library Preparation Kit, Illumina compatible. Fragments of corresponding size were size-selected using the agarose gel (1%) size selection protocol. Fragment size distribution was analyzed by the Agilent 2100 Bioanalyzer instrument using the High Sensitivity DNA Kit.A: DNA fragment library after size selection: 1 – 600-base reads, 2 – 200-base reads, 3 – 50-base reads; B: amplified DNA fragment library before size selection; C: DNA library after fragmentation

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