MagJET Plasmid DNA Kit 质粒提取试剂盒

货号：K2791,K2792

规格：96 preps,4 x 96 preps

价格：1233,4281

产品类型：DNA提取

品牌：Thermo Fisher

描述Thermo Scientific MagJET Plasmid DNA Kit, sufficient for 4 x 96 preps, is designed for fast and efficient purification of plasmid DNA (up to 4-5 µg/mL) or high copy plasmid DNA from overnight E. coliculture.the kit utilizes paramagnetic bead technology enabling high yields and robust performance. High binding capacity, uniform particle size, and rapid magnetic response of MagJET Magnetic Beads makes the technology ideal for high throughput automatic nucleic acid purification, as well as for manual purification for low sample throughput users.The resulting high quality DNA is free of proteins, nucleases and other contaminants or inhibitors. Purified plasmid DNA can be used in a wide range of downstream applications such as PCR, qPCR and other enzymatic reactions.Highlights•High yields– up to 5 µg of plasmid DNA from 1 mL of bacterial culture•Pure– isolated DNA is immediately ready-to-use in downstream application such as sequencing, transcription, digestion with restriction enzymes or transfection of robust cell lines•Flexible– automation and manual processing protocols available•Repeatable results and robust performanceApplications: Fast isolation of high purity plasmid DNA suitable for all conventional molecular biology procedures, including:• FastDigest or conventional restriction digestion• Automated fluorescent and radioactive sequencing• PCR•In vitrotranscription• Transformation• Transfection of robust cell linesHigh yields of purified plasmid DNAHigh-copy number plasmid DNA was isolated from overnight bacterial culture (1.5mL, Top10, DH5α and DH10B) using recommended Thermo Scientific protocol and protocols from competing kits. Purified plasmid DNA was analyzed by agarose (1%) electrophoresis.

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