ZO-1 Polyclonal Antibody/ZO-1多克隆抗体

货号：61-7300

规格：100 µg

价格：5508

产品类型：免疫组化一抗

品牌：Thermo Fisher

抗原：A 69 kD fusion protein(1) corresponding to amino a

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.25 mg/mL	用法：	Assay Dependent(ELISA)；5-10 µg/mL(ICC)；5-10 µg/mL(IF)；2-5 µg/mL(IHC)；Assay Dependent(IP)；0.5-2 µg/mL(WB)

产品详细信息61-7300 has been successfully used in Western blot, Immunoprecipitation, Immunofluorescence, ELISA and Immunohistochemistry.To achieve appropriate staining results on formalin-fixed, paraffin embedded tissue sections, protease Type XIV (Actinase E) pretreatment is required. The recommended dilution and incubation for protease is 1-2 mg/mL at 37°C for 10 minutes. Other enzyme and Heat Induced Epitope Retrieval pre-treatment methods may not be suitable for this particular antibody. Frozensections have been successfully stained after fixation with acetone at 4°C.靶标信息ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

ZO-1 Antibody (61-7300) in IFImmunofluorescent analysis of ZO-1 (green) in Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes and blocked with 3% Blocker BSA (Product # 37525) in PBS for 15 minutes at room temperature. Cells were stained with or without ZO-1 rabbit polyclonal antibody (Product # 61-7300), at a concentration of 5 µg/mL for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:1000 for 1 hour at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification. ZO-1 Antibody (61-7300) in IFIndirect immunofluorescence staining of rat glomerulus using Zymed (R) Rb anti-ZO-1 (Product # 61-7300) (red). Image courtesy of Hidetake Kurihara, PhD, Juntendo University School of Medicine, Tokyo, Japan. ZO-1 Antibody (61-7300) in IHCImmunohistochemical analysis of ZO-1 in small intestine using a polyclonal antibody (Product # 61-7300).

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参考文献：

1.Frontiers in neuroscienceProtective Effects of L-902,688, a Prostanoid EP4 Receptor Agonist, against Acute Blood-Brain Barrier Damage in Experimental Ischemic Stroke.2.Frontiers in microbiologyAfadin Downregulation byHelicobacter pyloriInduces Epithelial to Mesenchymal Transition in Gastric Cells.3.Asian journal of andrologyRegulation of blood-testis barrier dynamics by the mTORC1/rpS6 signaling complex: Anin vitrostudy.4.Journal of neurogastroenterology and motilityExpression of Neurotrophic Factors, Tight Junction Proteins, and Cytokines According to the Irritable Bowel Syndrome Subtype and Sex.5.Scientific reportsA Simulated Microgravity Environment Causes a Sustained Defect in Epithelial Barrier Function.

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