Occludin Polyclonal Antibody/闭合蛋白多克隆抗体

货号：71-1500

规格：100 µg

价格：4645

产品类型：免疫组化一抗

品牌：Thermo Fisher

抗原：A fusion protein containing the C-terminal 150 ami

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.25 mg/mL	用法：	Assay Dependent(ELISA)；2 µg/mL(ICC)；2 µg/mL(IF)；Assay Dependent(IHC)；Assay Dependent(IP)；1.5-2 µg/mL(WB)

靶标信息Occludin is a 65 kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82 kDa. Occludin is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution. Overall structural features of the occludin protein are highly conserved in all the species examined. Under-expression of tight junction proteins, including occludin, is a key molecular abnormality responsible for the increased permeability of tumor endothelial tight junctions, which contributes to brain tumor edemas.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

Occludin Antibody (71-1500) in IFImmunofluorescence analysis of Occludin was performed using 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Occludin Rabbit Polyclonal Antibody (Product # 71-1500) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cell junctional localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification. Occludin Antibody (71-1500) in IFMDCK canine cells stained with Rabbit anti-Occludin (Product # 71-1500) and Zymed's FITC-Goat Anti-Rabbit IgG (H+L) (Product # 62-6111). Occludin Antibody (71-1500) in IFImmunofluorescent analysis of Occludin using a polyclonal antibody (Product # 71-1500).

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参考文献：

1. Frontiers in microbiologyAfadin Downregulation byHelicobacter pyloriInduces Epithelial to Mesenchymal Transition in Gastric Cells.2. Oxidative medicine and cellular longevityMetformin Improves Fertility in Obese Males by Alleviating Oxidative Stress-Induced Blood-Testis Barrier Damage.3. Oncology reportsEffects of CCL5 on the biological behavior of breast cancer and the mechanisms of its interaction with tumor‑associated macrophages.4. Scientific reportsA Simulated Microgravity Environment Causes a Sustained Defect in Epithelial Barrier Function.5. ToxinsEffects of Claudin-1 on the Action ofClostridium perfringensEnterotoxin in Caco-2 Cells.

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