N-cadherin Monoclonal Antibody (3B9)/N-钙粘蛋白单克隆抗体（3B9）

货号：33-3900

规格：100 µg

价格：4713

产品类型：免疫组化一抗

品牌：Thermo Fisher

抗原：Recombinant domain corresponding to the intracellu

物种：其它

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：3B9

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	1-3 µg/mL(ICC)；1-3 µg/mL(IF)；Assay Dependent(IHC)；1-5 µg/mL(IHC (P))；3-5 µg(IP)；0.5-1 µg/mL(WB)

产品详细信息This antibody is specific for N-cadherin and does not cross-react with other cadherin family members including P- and E-cadherins.For IHC with this antibody use formalin-fixed, paraffin-embedded tissue and heat-induced epitope retrieval.靶标信息N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

N-cadherin Antibody (33-3900) in IFImmunofluorescent analysis of N-Cadherin was done on 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with N-Cadherin Mouse Monoclonal Antibody (Product # 33-3900) at 1 µg - 2 µg in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938)and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) panel c. Panel d is a merged image showing cell junctional localization of N-Cadherin. Panel e shows no primary antibody. The images were captured at 20X magnification. N-cadherin Antibody (33-3900) in IHCImmunohistochemistry analysis of N-Cadherin was done on human heart tissue section. The tissue was probed with N-cadherin Mouse Monoclonal Antibody (Product # 33-3900). N-cadherin Antibody (33-3900) in WBWestern blot analysis of total Cadherin and N-Cadherin was performed by loading 2 µL SeeBlue® Plus2 Prestained Protein Ladder (Product # LC5925), 50 µg of MDCK cell lysates and 10 µg mouse heart lysate per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 1% BSA/TBST for at least 1 hour at room temperature. Total cadherin was detected using a rabbit antibody (Product # 71-7100) and N-Cadherin was detected using a mouse antibody (Product # 33-3900), both at a concentration of 1 µg/mL in blocking buffer overnight at 4°C on a rocking platform. The blot was then incubated with goat anti-rabbit IgG-Alexa Fluor 790 secondary antibody (Product # A11369) and goat anti-mouse IgG-Alexa Fluor 680 secondary antibody (Product # A-21058) at a dilution of 1:10,000 for at least 1 hour. Fluorescent detection was performed using the Odyssey® CLx imaging system (Li-cor Biosciences). Image is generated by Joell Solan in Paul Lampe Lab at Fred Hutchinson Cancer Research Center.

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参考文献：

1 Stem cells internationalIsolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity.2 The Journal of clinical investigationAnkyrin-B dysfunction predisposes to arrhythmogenic cardiomyopathy and is amenable to therapy.3 The Journal of biological chemistryDefining new mechanistic roles for αII spectrin in cardiac function.4 Cell death and diseasePlanar cell polarity protein Dishevelled 3 (Dvl3) regulates ectoplasmic specialization (ES) dynamics in the testis through changes in cytoskeletal organization.5 JCI insightNitric oxide-sensitive guanylyl cyclase stimulation improves experimental heart failure with preserved ejection fraction.

技术参数

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