CD253 (TRAIL) Monoclonal Antibody (RIK-2), Functional Grade, eBioscience™/CD253 (TRAIL) 单克隆抗体 (RIK-2), 功能抗体

货号：16-9927-82,16-9927-025

规格：100 ug,25 mg

价格：1414,67500

产品类型：流式抗体

品牌：Alfa aesar

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

荧光染料：其它

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1.0µg/test（Flow）

Description: The RIK-2 monoclonal antibody reacts with human TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily. TRAIL is not expressed by resting human cells but has been shown to be induced under certain activation conditions. Iin addition it is expressed on several human tumor lines. Interaction of TRAIL with its ligand, Apo-2, induces apoptosis. RIK-2 blocks TRAIL-induced apoptosis.Applications Reported: The RIK-2 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of TRAIL in functional assays.Applications Tested: The RIK-2 antibody has been tested for blocking of staining with fluorochrome conjugated RIK-2 in flow cytometric analysis of human TRAIL transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TRAIL preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Receptors for TRAIL include two death domain containing receptors, death receptor 4 (DR4) and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain. TRAIL is a type II membrane protein and expressed in a variety of human tissues. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD253 (TRAIL) Antibody (16-9927-82) in FlowStaining of human Trail transfected cells with Anti-Human CD253 (TRAIL) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

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参考文献：

1.PLoS neglected tropical diseasesDengue virus activates membrane TRAIL relocalization and IFN-α production by human plasmacytoid dendritic cells in vitro and in vivo."16-9927 was used in Immunocytochemistry-immunofluorescence to characterise the activation of human plasmacytoid dendritic cells during DENV-2 infection in vivo, and their antiviral role in vitro."AuthorsGandini M,Gras C,Azeredo EL,Pinto LM,Smith N,Despres P,da Cunha RV,de Souza LJ,Kubelka CF,Herbeuval JP2.PloS oneMADD knock-down enhances doxorubicin and TRAIL induced apoptosis in breast cancer cells."16-9927 was used in Flow cytometry/Cell sorting to investigate how strategies to decrease MADD expression in breast cancer may be utilised to increase tumour cell sensitivity to TRAIL and doxorubicin induced apoptosis."AuthorsTurner A,Li LC,Pilli T,Qian L,Wiley EL,Setty S,Christov K,Ganesh L,Maker AV,Li P,Kanteti P,Das Gupta TK,Prabhakar BS3.BMC musculoskeletal disordersTRAIL death receptor-4, decoy receptor-1 and decoy receptor-2 expression on CD8+ T cells correlate with the disease severity in patients with rheumatoid arthritis."Published figure using CD253 (TRAIL) monoclonal antibody (Product # 16-9927-82) in Flow Cytometry"4.Proceedings of the National Academy of Sciences of the United States of AmericaHIV turns plasmacytoid dendritic cells (pDC) into TRAIL-expressing killer pDC and down-regulates HIV coreceptors by Toll-like receptor 7-induced IFN-alpha."16-9927 was used in Flow cytometry/Cell sorting to show that infectious and noninfectious HIV-1 virions induce activation of plasmacytoid dendritic cells (pDC) into TRAIL-expressing, IFN-producing killer pDCs."AuthorsHardy AW,Graham DR,Shearer GM,Herbeuval JP

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