Thermo Fisher ,Dynabeads™ Protein G for Immunoprecipitation/Dynabeads免疫沉淀蛋白G-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Thermo Fisher ,Dynabeads™ Protein G for Immunoprecipitation/Dynabeads免疫沉淀蛋白G

2024-10-24

Dynabeads™ Protein G for Immunoprecipitation/Dynabeads免疫沉淀蛋白G

货号：10003D,10004D,10009D

规格：1 mL,5 mL,50 mL

价格：2265,9239,69420

产品类型：免疫印迹/组化

品牌：Thermo Fisher

Dynabeads® 蛋白G是一种极佳的海藻糖和琼脂糖浆液替代物，可用于免疫沉淀。Dynabeads® 蛋白G广泛应用于小规模IgG纯化和抗体标记、免疫沉淀 (IP)、染色质免疫沉淀 (ChIP) 及蛋白质分离。磁性分离技术快速且温和，对靶蛋白的物理压力极小。因此可用于分离和浓缩易分解的成分，这些成分通常会在漫长的孵育过程中解离或被蛋白酶破坏。天然蛋白质的构象和完整的大蛋白复合体将受到保护。表面偶联有蛋白G的Dynabeads® 可分离大多数哺乳动物免疫球蛋白 (Ig)。参见下文图像和数据中的结合强度表，查看不同种属和亚类的结合强度。捕获的Ig量取决于起始样品中Ig的浓度以及Ig的类型和来源。该产品在可重复性和自动化能力方面秉承了Dynal的高标准，为您的分析提供了可靠性。

优点和特点：

• 不使用离心柱，无需离心或耗时的样品预处理• 小规模IgG纯化和免疫沉淀的完美选择• 方便的处理和简短的实验方案• 温和，对珍贵的蛋白质的物理压力极小• 微珠上的重组蛋白G不含有白蛋白结合位点，避免了残留蛋白质的共纯化

应用：

小规模IgG纯化、免疫沉淀 (IP)、染色质免疫沉淀 (ChIP) 及蛋白质分离。

数据：

Low non-specific binding with manual and automated immunoprecipitationImmunoprecipitation from HeLa cell lysate (Lysate) with Dynabeads™ Protein G bound to an irrelevant antibody (Ab) using either manual (Man) or automated protocol (Aut) on KingFisher™ Flex processor. Mild or denaturing elution conditions were followed by silver staining (SilverQuest™ Silver Staining Kit) of gels (Bolt™ 4-12% Bis Tris Plus). Equally low non-specific binding was seen with both the automated and manual protocols for both elution conditions.

Manual and automated immunoprecipitation of CD9Immunoprecipitation of CD9 from HeLa cell lysate with Dynabeads™ Protein G bound to anti-human CD9 using either manual (Man) or automated (Aut) protocol on KingFisher™ Flex processor. The purified CD9 protein was detected using SDS gel electrophoresis (Bolt™ 4-12% Bis Tris Plus) followed by western blot (Bolt™ Western Pack B). CD9 was detected using anti-CD9 (Cat. No. 10626D). The western blot shows that the CD9 protein is purified just as efficiently with the automated protocol as with the manual protocol. (Ladder: SeeBlue™ Plus2 Pre-stained Protein Standard.)

Comparison of results for all methods vs. Dynabeads® Protein G

技术参数

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Thermo Fisher ,Dynabeads™ Protein G for Immunoprecipitation/Dynabeads免疫沉淀蛋白G

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