CD284 (TLR4) Monoclonal Antibody (HT52), Functional Grade, eBioscience™/CD284 (TLR4) 单克隆抗体 (HT52), 功能抗体

货号：16-9879-85

规格：500 µg

价格：5144

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

荧光染料：Alexa Fluor 488

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.25 µg/test（Flow）

产品详细信息Description: The HT52 monoclonal antibody reacts with human Toll-like receptor (TLR) 4. Thus far, at least ten members of the Toll family have been identified in humans. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two family members, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins; they have beenimplicated in innate immunity and inflammation. TLR4 physically associates with another molecule called MD-2. Together with CD14, this complex is responsible for LPS recognition and signaling. TLR4 is expressed by peripheral blood monocytes. Most TLR cell surface expression, especially TLR1 and TLR4, occurs at low levels on monocytes and at even lower levels on other cell types, including granulocytes and immature dendritic cells (iDCs). Furthermore, a relatively high degree of variability in TLR surface expression has been reported among normal donors.The HT52 antibody has been reported to exhibit neutralizing activity.Applications Reported: This HT52 antibody has been reported for use in functional assays and flow cytometric analysis.Applications Tested: This HT52 antibody has been tested by flow cytometric analysis of TLR-4-transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.仅用于科研。不用于诊断过程。未经明确授权不得转售

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参考文献：

1.Journal of virologyHepatitis B Virus Surface Antigen Activates Myeloid Dendritic Cells via a Soluble CD14-Dependent Mechanism."16-9879 was used in Neutralization experiments to identify CD14 and TLR4 as receptors for HBsAg, the main HBV envelope antigen."Authorsvan Montfoort N,van der Aa E,van den Bosch A,Brouwers H,Vanwolleghem T,Janssen HLA,Javanbakht H,Buschow SI,Woltman AM2.Frontiers in immunologyLipopolysaccharide induces IFN-γ production in human NK cells."16-9879 was used in Flow cytometry/Cell sorting to suggest the existence of a mechanism of LPS direct action on NK cells distinct from established TLR4-mediated signaling."AuthorsKanevskiy LM,Telford WG,Sapozhnikov AM,Kovalenko EI

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