CD283 (TLR3) Monoclonal Antibody (TLR3.7), Functional Grade, eBioscience™/CD283 (TLR3) 单克隆抗体(TLR3.7), 功能级, eBioscience™

货号：16-9039-82

规格：100 µg

价格：3386

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

荧光染料：其它

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	2.0µg/test（Flow）

产品详细信息Description: The TLR3.7 monoclonal antibody reacts with human Toll-like receptor 3 (TLR3). To date, at least twelve members of the Toll-like receptor family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. In the innate immune response, TLRs recognize molecular patterns associated with microbial pathogens and induce antimicrobial activity. TLR3 recognizes double-stranded (ds)RNA, induces the activation of NF-kappaB through MyD88-dependent and -independent pathways, and the production of type I interferons (IFNs). TLR3.7 suppressed poly(I):poly(C)-mediated IFN-beta production by human fibroblasts naturally expressing TLR3 on their surface.Applications Reported: The TLR3.7 antibody has been reported for use in flow cytometric analysis. It has also been reported in in vitro inhibition of ligand binding. For optimal detection of TLR3 with this mAb by flow cytometry, PE-conjugated or Biotin-conjugated TLR3.7 (revealed with SAv-PE cat 12-4312) are recommended.Applications Tested: This TLR3.7 antibody has been tested by intracellular staining and flow cytometric analysis of A549 and MRC5 cell lines to detect the very low level of this antigen on the surface. This can be used at less than or equal to 2 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.torage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息TLR3 (Toll-like receptor 3, CD283) is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from drosophila to humans and share structural and functional similarities. TLRs recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR3 is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. TLR3 recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. Further, TLR3 may play a role in host defense against viruses, and the use of alternative polyadenylation sites to generate different length transcripts has been noted for the TLR3 gene. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. TLR3 is the only human TLR which does not utilize MyD88 as an adaptor molecule instead utilizing TRIF in a signaling path which results in downstream activation of IRF-3 and NF-kB. TLR3 activation leads not only to Type I interferon induction but also to other inflammatory cytokines which result in DC maturation. TLR3 is known to recognize viral double-stranded (ds) RNA, a molecular pattern associated with viral infection. Recently it has been shown to recognize viruses such as Influenza A and West Nile Virus and can mediate entry of at least West Nile Virus.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD283 (TLR3) Antibody (16-9039-82) in FlowStaining of permeabilized A549 cells with 1 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 1 µg of Anti-Human CD283 (TLR3) Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Total cells were used for analysis.

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参考文献：

1.Scientific reportsA potential mode of action for Anakinra in patients with arthrofibrosis following total knee arthroplasty."Published figure using CD283 (TLR3) monoclonal antibody (Product # 16-9039-82) in Flow Cytometry"2.Human immunologyHuman endometrial epithelial cells cyclically express Toll-like receptor 3 (TLR3) and exhibit TLR3-dependent responses to dsRNA.3.Journal of leukocyte biologyAn immunomodulatory protein, Ling Zhi-8, induced activation and maturation of human monocyte-derived dendritic cells by the NF-kappaB and MAPK pathways."16-9039 was used in Neutralization experiments to demonstrate that rLZ-8 can activate and maturate immature DCs, via Th1 response, suggesting that rLZ-8 may potentially regulate immune responses."AuthorsLin YL,Liang YC,Tseng YS,Huang HY,Chou SY,Hseu RS,Huang CT,Chiang BL4.FASEB journal : official publication of the Federation of American Societies for Experimental BiologyExpression of toll-like receptors by human muscle cells in vitro and in vivo: TLR3 is highly expressed in inflammatory and HIV myopathies, mediates IL-8 release and up-regulation of NKG2D-ligands.AuthorsSchreiner B,Voss J,Wischhusen J,Dombrowski Y,Steinle A,Lochmüller H,Dalakas M,Melms A,Wiendl H5.Human immunologyHuman endometrial epithelial cells cyclically express Toll-like receptor 3 (TLR3) and exhibit TLR3-dependent responses to dsRNA.

技术参数

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