CD284 (TLR4) Monoclonal Antibody (HTA125), Functional Grade, eBioscience™/CD284 (TLR4) 单克隆抗体 (HTA125), 功能抗体

货号：16-9917-82,16-9917-38

规格：100 µg,5 mg

价格：2654,24089

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

荧光染料：其它

类型:	一抗	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1.0µg/test（Flow）

产品详细信息Description: The HTA125 monoclonal antibody reacts with human Toll-like receptor 4 (TLR4). So far, at least ten members of the Toll family have been identified in humans. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with another molecule called MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. TLR4 is expressed by peripheral blood monocytes. HTA125 has been reported to immunoprecipitate human TLR4 (~100 kDa) from transfected cells. Most TLR cell surface expression, especially TLR1 and TLR4, occurs at low levels on monocytes and at even lower levels on other cell types including granulocytes and immature dendritic cells (iDC). Furthermore, a relatively high degree of variability in TLR surface expression has been reported among normal donors.It is highly recommended that for optimal staining of TLR4, whole blood be stained using the lysed whole blood protocol (found inBest Protocols) rather than Ficoll-gradient prepared normal human peripheral blood cells. The use of a density gradient appears to reduce the staining intensity significantly.Applications Reported: The HTA125 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of LPS-induced cytokine production. For detection of peripheral monocytes, a three step staining protocol is recommended using purified anti-human TLR4 followed by biotin anti-mouse IgG and streptavidin-PE.Applications Tested: The HTA125 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.It is highly recommended that for optimal staining of TLR4, whole blood be stained using the LWB protocol found in Best Protocols rather than Ficoll-gradient prepared normal human peripheral blood cells. The use of a density gradient appears to reduce the staining intensity significantly.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD284 (TLR4) Antibody (16-9917-82) in FlowStaining of normal human peripheral blood cells with Mouse IgG2a Isotype Control (Product # 16-4724-85) or Anti-Human CD284 (TLR4) Functional Grade Purified. Cells in the monocyte population were used for analysis.

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参考文献：

1.International journal of molecular medicineBacterial lipopolysaccharide and antimicrobial LL-37 enhance ICAM-1 expression and NF-κB p65 phosphorylation in senescent endothelial cells."Published figure using CD284 (TLR4) monoclonal antibody (Product # 16-9917-82) in Flow Cytometry"2.Oncology lettersButyrate upregulates the TLR4 expression and the phosphorylation of MAPKs and NK-κB in colon cancer cellin vitro."Published figure using CD284 (TLR4) monoclonal antibody (Product # 16-9917-82) in Flow Cytometry"3.OncotargetAltered Toll-like receptor expression and function in HPV-associated oropharyngeal carcinoma."Published figure using CD284 (TLR4) monoclonal antibody (Product # 16-9917-82) in Flow Cytometry"4.Microbiology (Reading, England)The uptake of a Klebsiella pneumoniae capsule polysaccharide mutant triggers an inflammatory response by human airway epithelial cells."16-9917 was used in Flow cytometry/Cell sorting to indicate that Klebsiella pneumoniae capsule polysaccharide internalization by airway epithelial cells could trigger activation of the innate immune system."AuthorsRegueiro V,Campos MA,Pons J,Albertí S,Bengoechea JA

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