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产品介绍
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Rat anti Mouse CD19 antibody, clone 6D5recognizes the murine CD19 cell surface antigen, a ~95 kDa glycoprotein expressed by B lymphocytes. Rat anti Mouse CD19 antibody, clone 6D5 recognizes the same, or a closely related epitope as clone Rat anti Mouse CD19 antibody, clone ID3 in cross-competition assays.
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产品详情
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Target Species
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Mouse
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Product Form
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Purified IgG conjugated to Alexa Fluor® 700 - liquid
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Preparation
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Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
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BufferSolution
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Phosphate buffered saline
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Preservative Stabilisers
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0.09% Sodium Azide
1% Bovine Serum Albumin
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Immunogen
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Human K562 cell line transfected with murine CD19.
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Approx. Protein Concentrations
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IgG concentration 0.05 mg/ml
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Fusion Partners
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Spleen cells from immunised rats were fused with cells of the P3X63.Ag8.653 myeloma cell line.
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Max Ex/Em
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Fluorophore
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Excitation Max (nm)
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Emission Max (nm)
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Alexa Fluor®700
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702
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723
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Regulatory
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For research purposes only
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Guarantee
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12 monthsfrom date of despatch
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Acknowledgements
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This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research.
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存储条件
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This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
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应用
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Application Name
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Verified
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Min Dilution
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Max Dilution
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Flow Cytometry
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√
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Neat
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1/10
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This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 1 x106cells in 100ul.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
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同型
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Description
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Product Code
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Applications
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Pack Size
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Rat IgG2a Negative Control:Alexa Fluor® 700
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MCA1212A700
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F
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100 Tests/1ml
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文献
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1.Starck, J.et al.(2010) Inducible Fli-1 gene deletion in adult mice modifies several myeloid lineage commitment decisions and accelerates proliferation arrest and terminal erythrocytic differentiation.Blood. 116: 4795-805.2. Scotland, R.S.et al.(2011) Sex differences in resident immune cell phenotype underlie more efficient acute inflammatory responses in female mice.Blood. 118 (22): 5918-27.3. White, H.N. and Meng, Q.H. (2012) Recruitment of a Distinct but Related Set of VH Sequences into the Murine CD21hi/CD23- Marginal Zone B Cell Repertoire to That Seen in the Class-Switched Antibody Response.J Immunol. 188: 287-93.4. Reynaud, J.M.et al.(2014) Human herpesvirus 6A infection in CD46 transgenic mice: viral persistence in the brain and increased production of proinflammatory chemokines via Toll-like receptor 9.J Virol. 88: 5421-36.5. Candolfi, M.et al.(2011) B cells are critical to T-cell-mediated antitumor immunity induced by a combined immune-stimulatory/conditionally cytotoxic therapy for glioblastoma.Neoplasia. 13: 947-60.6. Takabayashi, H.et al.(2014) Anti-inflammatory activity of bone morphogenetic protein signaling pathways in stomachs of mice.Gastroenterology. 147: 396-406.e7.7. Weiss-Gayet, M.et al.(2016) Notch Stimulates Both Self-Renewal and Lineage Plasticity in a Subset of Murine CD9High Committed Megakaryocytic Progenitors.PLoS One. 11 (4): e0153860.8. Meng, Q.H. & White, H.N. (2017) CD21intCD23+follicular B cells express antigen-specific secretory IgM mRNA as primary and memory responses.Immunology. 151 (2): 211-8.9. Mccubbrey, A.L.et al.(2016) MicroRNA-34a Negatively Regulates Efferocytosis by Tissue Macrophages in Part via SIRT1.J Immunol. 196 (3): 1366-75.10. Vila-Caballer, M.et al.(2019) Disruption of the CCL1-CCR8 axis inhibits vascular Treg recruitment and function and promotes atherosclerosis in mice.J Mol Cell Cardiol. 132: 154-63.
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QQ:1749072012